P8999

モノクロナール抗p53 マウス宿主抗体

Name: モノクロナール抗p53 マウス宿主抗体
Brand: SIGMA

clone DO-7, purified from hybridoma cell culture

別名:

抗TRP53抗体, 抗形質転換関連タンパク質53抗体

ログインで組織・契約価格をご覧ください。

サイズを選択してください

この商品について

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164709

Conjugate:

unconjugated

Clone:

DO-7, monoclonal

Application:

ELISA (i), ICC, IHC, IP, WB

Citations:

主要文書

すべての文書を表示

テクニカルサービス

お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。

お手伝いします

テクニカルサービス

お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。

お手伝いします

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DO-7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.1-0.2 μg/mL using human A431

isotype

IgG2b

UniProt accession no.

P04637

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TP53(7157)

類似した製品をお探しですか？訪問製品比較ガイド

General description

Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues.

Application

Monoclonal Anti-p53 antibody produced in mouse has been used in:

immunoblotting
enzyme linked immunosorbent assay (ELISA)
immunocytochemistry
immunohistochemistry
immunoprecipitation

Biochem/physiol Actions

Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest.

p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

適切な製品が見つかりませんか。

製品選択ツール.をお試しください

保管分類

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

P8999-BULK: + P8999-200UL: + P8999-100UL: + P8999-25UL: + P8999-VAR:

jan

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

COAを検索

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Role of p53, apoptosis, and cell proliferation in early stage Epstein-Barr virus positive and negative gastric carcinomas

Ishii HH, et al.

Journal of Clinical Pathology, 57(12), 1306-1311 (2004)

Chk2/hCds1 functions as a DNA damage checkpoint in G1 by stabilizing p53

Chehab NH, et al.

Genes & Development, 14(3), 278-288 (2000)

DNA damage-induced activation of p53 by the checkpoint kinase Chk2

Hirao A, et al.

Science (New York, N.Y.), 287(5459), 1824-1827 (2000)

New Insights into p53 Signaling and Cancer Cell Response to DNA Damage: Implications for Cancer Therapy.

Mirzayans, R., et al.

Journal of Biomedicine and Biotechnology, doi: 10-doi: 10 (2012)

Bacterial Carriers for Glioblastoma Therapy.

Nalini Mehta et al.

Molecular therapy oncolytics, 4, 1-17 (2017-03-28)

Treatment of aggressive glioblastoma brain tumors is challenging, largely due to diffusion barriers preventing efficient drug dosing to tumors. To overcome these barriers, bacterial carriers that are actively motile and programmed to migrate and localize to tumor zones were designed.

資料

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

モノクロナール抗p53 マウス宿主抗体

clone DO-7, purified from hybridoma cell culture

サイズを選択してください

この商品について

主要文書

特性

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

concentration

technique(s)

isotype

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

関連するカテゴリー

詳細

General description

Application

Biochem/physiol Actions

Physical form

Disclaimer

安全性情報

保管分類

wgk

flash_point_f

flash_point_c

ppe

適用法令

P8999-BULK: + P8999-200UL: + P8999-100UL: + P8999-25UL: + P8999-VAR:

資料

試験成績書（COA）

以前この製品を購入いただいたことがある場合

参考文献

プロトコルと関連資料

資料

関連コンテンツ

テクニカルサービス