Quality Level
form
powder
solubility
water: 10 mg/mL, clear to very slightly hazy (faint yellow to light yellow)
suitability
suitable for (suitable for use as a substrate for Rnase)
storage temp.
−20°C
類似した製品をお探しですか？ 訪問 製品比較ガイド
関連するカテゴリー
Application
パン酵母（S. cerevisiae）由来リボ核酸（RNA）は、ノーザンブロットハイブリダイゼーション、逆転写ポリメラーゼ連鎖反応（RTPCR）、およびcDNA作製などの用途に適しています。’
General description
パン酵母（S. cerevisiae）由来のリボ核酸（RNA）は、RNase酵素の基質です。’ 酵母からのRNAの単離は複雑なプロセスで、フェニルや界面活性剤（SDS）の存在下で細胞を何回も加熱したり凍結したりします。
Preparation Note
Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955)の変法にて調製しています。
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Kazuya Shirato et al.
Virology journal, 11, 139-139 (2014-08-12)
The first documented case of Middle East Respiratory Syndrome coronavirus (MERS-CoV) occurred in 2012, and outbreaks have continued ever since, mainly in Saudi Arabia. MERS-CoV is primarily diagnosed using a real-time RT-PCR assay, with at least two different genomic targets
Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Radha Duttagupta et al.
PloS one, 7(2), e31241-e31241 (2012-02-24)
Inflammatory Bowel Disease--comprised of Crohn's Disease and Ulcerative Colitis (UC)--is a complex, multi-factorial inflammatory disorder of the gastrointestinal tract. In this study we have explored the utility of naturally occurring circulating miRNAs as potential blood-based biomarkers for non-invasive prediction of
Stephanie C Bannister et al.
Biology of reproduction, 81(1), 165-176 (2009-04-10)
MicroRNAs are a highly conserved class of small RNAs that function in a sequence-specific manner to posttranscriptionally regulate gene expression. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryogenesis, but a role for miRNAs
Douglas A Weigent
Cellular immunology, 282(2), 71-78 (2013-06-19)
In the present study, we show that higher levels of lymphocyte GH are expressed in spleen cells from aging animals compared to young animals. Further, leukocytes from primary and secondary immune tissues and splenic T and B cells from aging
プロトコル
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）