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Merck

R9778

モノクローナル抗hnRNP-A1抗体 マウス宿主抗体

~2 mg/mL, clone 4B10, purified from hybridoma cell culture

別名:

Anti-Heterogeneous Nuclear Ribonucleoprotein-A1

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD06798702
Conjugate:
unconjugated
Clone:
4B10, monoclonal
Application:
immunocytochemistry
immunoprecipitation (IP)
indirect ELISA
microarray
western blot
Species reactivity:
bovine, human, canine
Citations:
11
Technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of HeLa cells
Uniprot accession no.:
P09651

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製品名

モノクローナル抗hnRNP-A1抗体 マウス宿主抗体, ~2 mg/mL, clone 4B10, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

4B10, monoclonal

form

buffered aqueous solution

mol wt

antigen 32-35 kDa

species reactivity

bovine, human, canine

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of HeLa cells

isotype

IgG2a

UniProt accession no.

P09651

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... HNRNPA1(3178)

関連するカテゴリー

Immunogen

精製ヒトhnRNP-A1。

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-hnRNP-A1 antibody produced in mouse has also been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry.

Biochem/physiol Actions

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP-A1) is important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP-A1 from transportin. Higher expression is observed in proliferating and/or transformed cells than in differentiated tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-hnRNP-A1 (mouse IgG2a isotype) is derived from the 4B10 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from NZB mice immunized with purified human hnRNPA1. Heterogeneous nuclear ribonucleoprotein A1 (hnRNPs) consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa. hnRNP-A1 is ubiquitously expressed in cells and tissues.

Physical form

Solution in 0.01 M phosohate buffered saline, pH 7.4, and 15 mM sodium azide.

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保管分類

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

R9778-200UL: + R9778-BULK: + R9778-VAR:

jan

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試験成績書（COA）

Lot/Batch Number
0000223748
0000223748
0000207701
0000207701
055M4821V

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Youn-Jae Kim et al.
PloS one, 6(12), e28308-e28308 (2011-12-14)
Aberrant miR-21 expression is closely associated with cell proliferation, anti-apoptosis, migration, invasion, and metastasis in various cancers. However, the regulatory mechanism of miR-21 biogenesis is largely unknown. Here, we demonstrated that the tumor suppressor PTEN negatively regulates the expression of
hnRNP A1 nucleocytoplasmic shuttling activity is required for normal myelopoiesis and BCR/ABL leukemogenesis
Lervolino A, et al.
Molecular and Cellular Biology, 22(7), 2255-2266 (2002)
Functional diversity of the hnRNPs: past, present and perspectives
Han SP, et al.
The Biochemical Journal, 430(3), 379-392 (2010)
Blocking of an intronic splicing silencer completely rescues IKBKAP exon 20 splicing in familial dysautonomia patient cells
Bruun GH, et al.
Nucleic Acids Research, 46(15), 7938-7952 (2018)
Antisense Oligonucleotide Rescue of Deep-Intronic Variants Activating Pseudoexons in the 6-Pyruvoyl-Tetrahydropterin Synthase Gene.
Mart?-nez-Pizarro, et al.
Nucleic Acid Therapeutics, 32, 378-390 (2022)
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