S8447

抗Sirt2 ウサギ宿主抗体

Name: 抗Sirt2 ウサギ宿主抗体
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

別名:

抗SIR2L2抗体, 抗SIR2L抗体, 抗サーチュイン (silent mating type information regulation2ホモログ) 抗体, 抗サーチュイン 2抗体

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この商品について

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD07784768

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

主要文書

すべての文書を表示

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 37:43 kDa (rat), antigen 43 kDa (human)

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

technique(s)

immunoprecipitation (IP): 2-5 μg using extract of COS7 cells or 293 cells expressing recombinant human SIRT2 protein., indirect immunofluorescence: 5-10 μg/mL using human HeLa cells., western blot: 1-2 μg/mL using whole extracts of mouse and rat brain.

UniProt accession no.

Q8IXJ6

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... SIRT2(22933)
mouse ... Sirt2(64383)
rat ... Sirt2(361532)

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General description

Rabbit polyclonal anti-Sirt2 antibody recognizes mouse, rat (37/43 kDa), and human (43 kDa) Sirt2. Detection of the Sirt2 bands by immunoblotting is specifically inhibited with the immunizing peptide.

Sirt2 is an NAD+-dependent tubulin deacetylase. Two Sirt2 isoforms exist as the result of alternative splicing. Sirt2 is overexpressed during mitosis and is multiply phosphorylated at the G2/M transition of the cell cycle, indicating that it plays a role in the control of mitotic exit in the cell cycle. Although Sirt2 resides predominantly in the cytoplasm, it becomes enriched in the nucleus during the cell cycle where it is associated with mitotic structures. Sirt2 was found to be an oligodendroglial protein that affects cell differentiation through deacetylating α-tubulin.

Sirtuin 2 (SIRT2) is a cytoplasmic protein, which is part of the sirtuin family of proteins. SIRT2 colocalizes with microtubules. This gene is located on human chromosome 19q13.2.

Immunogen

マウスSirt2のアミノ酸341~352に相当する合成ペプチドのKLH結合体。相当する配列はラットと同一であり、ヒトSirt2では1アミノ酸が異なっています。

Application

Anti-Sirt2 antibody has been used in western blotting and immunofluorescence.

Rabbit polyclonal anti-Sirt2 antibody may be used in several applications including immunoblotting, immunoprecipitation, and immunofluorescence.

Biochem/physiol Actions

Sirtuin 2 (SIRT2) maintains the integrity of the genome. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington′s disease. SIRT2 can deacetylate lys40 of α-tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation.

Physical form

0.01 M PBS溶液 (pH 7.4, 15 mM アジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類

10 - Combustible liquids

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

S8447-25UL: + S8447-BULK: + S8447-100UL: + S8447-200UL: + S8447-VAR:

jan

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number

0000527525

0000445894

0000445895

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特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

COAを検索

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

SIRT2 ablation has no effect on tubulin acetylation in brain, cholesterol biosynthesis or the progression of Huntington's disease phenotypes in vivo

Bobrowska A, et al.

Testing, 7(4), e34805-e34805 (2012)

Expression of Sirtuins in the Retinal Neurons of Mice, Rats, and Humans.

Hongdou Luo et al.

Frontiers in aging neuroscience, 9, 366-366 (2017-12-19)

Sirtuins are a class of histone deacetylases (HDACs) that have been shown to regulate a range of pathophysiological processes such as cellular aging, inflammation, metabolism, and cell proliferation. There are seven mammalian Sirtuins (SIRT1-7) that play important roles in stress

SIRT2 inhibition achieves neuroprotection by decreasing sterol biosynthesis

Luthi-Carter R, et al.

Proceedings of the National Academy of Sciences of the USA, 201002924-201002924 (2010)

Sirtuin 2 mutations in human cancers impair its function in genome maintenance

Head P E, et al.

The Journal of biological chemistry, jbc-M116 (2017)

SIRT2 is required for efficient reprogramming of mouse embryonic fibroblasts toward pluripotency

Kim A Y, et al.

Cell Death & Disease, 9(9), 893-893 (2018)

抗Sirt2 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

サイズを選択してください

この商品について

主要文書

特性

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

packaging

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

関連するカテゴリー

詳細

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Disclaimer

安全性情報

保管分類

ppe

適用法令

S8447-25UL: + S8447-BULK: + S8447-100UL: + S8447-200UL: + S8447-VAR:

資料

試験成績書（COA）

以前この製品を購入いただいたことがある場合

参考文献

プロトコルと関連資料

関連コンテンツ

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