SAB4200400

Anti-WIPI-2 (C-terminal) antibody produced in rabbit

Name: Anti-WIPI-2 (C-terminal) antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody

別名:

Anti-ATG18B, Anti-Atg21, Anti-CGI-50, Anti-WD repeat domain, phosphoinositide interacting 2, Anti-WIPI2

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この商品について

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB

Citations:

主要文書

すべての文書を表示

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concentration

~1.0 mg/mL

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~49 kDa

species reactivity

mouse, rat, human

technique(s)

immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells., western blot: 2-4 μg/mL using whole extracts of human G-361 and NIH-3T3 cells.

UniProt accession no.

Q9Y4P8

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... WIPI2(26100)
mouse ... Wipi2(74781)
rat ... Wipi2(288498)

General description

WIPI-2 belongs to the WIPI subfamily of WD40 repeat proteins. It is widely expressed in a variety of cell lines. The protein is characterized with a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. It is a membrane constituent of autophagosomes and the plasma membrane.

Immunogen

synthetic peptide corresponding to the C-terminal region of human WIPI-2, conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Application

Anti-WIPI-2 (C-terminal) antibody produced in rabbit may be used in various immunochemical techniques including

immunoblotting
immunoprecipitation
immunostaining.

Biochem/physiol Actions

WIPI subfamily proteins acts as a key component of many essential biological functions such as signal transduction, transcription regulation and apoptosis. WIPI-2 is a phosphatidylinositol-3-phosphate binding protein that is needed for starvation induced autophagy. In addition, it is also essential for LC3 lipidation and it is linked to early autophagosomal structures together with Atg16L and ULK1.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

IXO15053: + SAB4200400-BULK: + SAB4200400-200UL: + SAB4200400-VAR:

jan

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試験成績書（COA）

Lot/Batch Number

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特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

COAを検索

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets

Tamura N, et al.

Febs Letters, 591(23), 3819-3830 (2017)

Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17

Matsui T, et al.

The Journal of cell biology, 217(8), 2633-2645 (2018)

Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes.

Proikas-Cezanne T

Journal of Cellular and Molecular Medicine, 15(9), 2007-2010 (2011)

Atg9A trafficking through the recycling endosomes is required for autophagosome formation.

Kenta Imai et al.

Journal of cell science, 129(20), 3781-3791 (2016-09-03)

Autophagy is an intracellular degradation pathway conserved in eukaryotes. Among core autophagy-related (Atg) proteins, mammalian Atg9A is the sole multi-spanning transmembrane protein, and both of its N- and C-terminal domains are exposed to the cytoplasm. It is known that Atg9A

Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.

Polson HE

Autophagy, 6(4), 506-522 (2010)

Anti-WIPI-2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

サイズを選択してください

この商品について

主要文書

特性

concentration

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Gene Information

詳細

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Disclaimer

安全性情報

保管分類

flash_point_f

flash_point_c

適用法令

IXO15053: + SAB4200400-BULK: + SAB4200400-200UL: + SAB4200400-VAR:

資料

試験成績書（COA）

以前この製品を購入いただいたことがある場合

参考文献

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