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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
KN-4, monoclonal
Application:
IF, WB
Citations:
9
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
KN-4, monoclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa (isoform 4), antigen ~60 kDa (isoform 2), antigen ~77 kDa (isoform 1)
species reactivity
mouse, human
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 2.5-5.0 μg/mL using SW-620 cells, western blot: 1.0-2.0 μg/mL using SW-48 total cell extracts
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FERMT1(55612)
mouse ... Fermt1(241639)
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General description
Kindlin-1 is coded by FERMT1 (fermitin family member 1) gene. It is a focal adhesion protein, expressed in the skin and intestine. FERMT1 is located on human chromosome 20p.12.3.
Immunogen
synthetic peptide corresponding to a sequence close to the N-terminus of human Kindlin-1, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Kindlin-1 antibody has been used in immunohistochemistry, immunofluorescence staining and western blotting.
Biochem/physiol Actions
Kindlin-1 protein, coded by FERMT1 gene modulates keratinocyte electrotaxis. It participates in the initiation of the integrin family of extracellular matrix receptors. FERMT1 maintains the lamellipodial protrusions at the time of electrotaxis. Mutations in kindlin-1 results in Kindler syndrome (KS).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
SAB4200465-200UL: + SAB4200465-VAR: + SAB4200465-BULK:
jan
Kindlin-2 controls TGF-beta signalling and Sox9 expression to regulate chondrogenesis
Wu CJ, et al.
Nature Communications, 6(4), 7531-7531 (2015)
Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing
Shen CS, et al.
International Journal of Molecular Medicine, 39(4), 949-959 (2017)
Case of Kindler syndrome resulting from mutation in the FERMT1 gene
Wada MM, et al.
The Journal of Dermatology, 39(12), 1057-1058 (2012)
Kindlin-1 regulates keratinocyte electrotaxis
Zhang G G, et al.
The Journal of Investigative Dermatology, 136(11), 2229-2239 (2016)
New intragenic and promoter region deletion mutations in FERMT 1 underscore genetic homogeneity in Kindler syndrome
Fuchs-Telem D, et al.
Clinical and Experimental Dermatology, 39(3), 361-367 (2014)
関連コンテンツ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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