SAB4200661
Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse
clone DYN2-11, purified from hybridoma cell culture
別名:
CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5, dynamin 2
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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
DYN2-11
Application:
IF, IP
Citations:
8
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
DYN2-11
form
buffered aqueous solution
mol wt
antigen ~98 kDa
species reactivity
mouse, human, monkey, canine, rat
concentration
~1 mg/mL
technique(s)
immunoblotting: 2-4 μg/mL using using whole extract of HeLa cells., immunofluorescence: 5-10 μg/mL using using HeLa cells., immunoprecipitation (IP): 5-10 μg using using whole extract of HeLa cells.
isotype
IgG1
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DNM2(1785)
mouse ... Dnm2(13430)
rat ... Dnm2(25751)
General description
Monoclonal Anti-Dynamin 2 (DNM2) (mouse IgG1 isotype) is derived from the hybridoma DYN2-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Dynamin 2 (DNM2) also known as DYN2 belongs to the dynamins family of guanosine triphosphatase (GTPase) proteins.
The gene DNM2 (dynamin 2) is mapped to human chromosome 19p13. It is widely expressed. The protein has a catalytic amino-terminal GTPase domain, a middle domain, a pleckstrin homology domain (PH), a GTPase effector domain (GED) and a less conserved carboxyl-terminal proline/arginine rich domain (PRD).
Immunogen
synthetic peptide corresponding to a sequence at the N-terminal region of human DNM2 , conjugated to KLH
Application
Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
DNM2 (dynamin 2) is a GTPase which is required for clathrin-mediated endocytosis. It is important for clathrin-coated pit maturation and clathrin-coated vesicle formation. DNM2 participates in neuronal morphology, axonal growth, centrosome cohesion, actin- and microtubular organization. Mutations in it are associated with Charcot-Marie-Tooth disease, centronuclear myopathy ADCNM (autosomal dominant centronuclear myopathy) and lethal congenital contractures syndrome type 5 (LCCS5). It is upregulated in prostate cancer.
Furthermore, DNM2 is upregulated in pancreatic cancer as it activates Ras-related C3 botulinum toxin substrate 1 (Rac1). It also promotes invasive cellular migration and lamellipod protrusion.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
SAB4200661-VAR: + SAB4200661-100UL: + SAB4200661-BULK:
jan
Riad Efendiev et al.
The Journal of biological chemistry, 277(46), 44108-44114 (2002-09-03)
Clathrin-dependent endocytosis of Na(+),K(+)-ATPase in response to dopamine regulates its catalytic activity in intact cells. Because fission of clathrin-coated pits requires dynamin, we examined the mechanisms by which dopamine receptor signals promote dynamin-2 recruitment and assembly at the site of
Gonçalo C Pereira et al.
PloS one, 15(6), e0234653-e0234653 (2020-06-25)
We previously demonstrated that hexokinase II (HK2) dissociation from mitochondria during cardiac ischemia correlates with cytochrome c (cyt-c) loss, oxidative stress and subsequent reperfusion injury. However, whether HK2 release is the primary signal mediating this ischemia-induced mitochondrial dysfunction was not
Gina L Razidlo et al.
Developmental cell, 24(6), 573-585 (2013-03-30)
The large GTPase Dynamin 2 (Dyn2) is markedly upregulated in pancreatic cancer, is a potent activator of metastatic migration, and is required for Rac1-mediated formation of lamellipodia. Here we demonstrate an unexpected mechanism of Dyn2 action in these contexts via
Bin Xu et al.
Cancer medicine, 3(1), 14-24 (2014-01-10)
Dynamin 2 (Dyn2) is essential for intracellular vesicle formation and trafficking, cytokinesis, and receptor endocytosis. In this study, we investigated the implication of Dyn2 as a prognostic marker and therapeutic target for progressive prostate cancer (PCA). We evaluated Dyn2 protein
Nyamkhishig Sambuughin et al.
BMC neurology, 15, 223-223 (2015-11-01)
Hereditary Spastic Paraplegia (HSP) represents a large group of clinically and genetically heterogeneous disorders linked to over 70 different loci and more than 60 recognized disease-causing genes. A heightened vulnerability to disruption of various cellular processes inherent to the unique
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