SAE0066
Adenylyl Cyclase Toxin from Bordetella pertussis
ログインで組織・契約価格をご覧ください。
サイズを選択してください
この商品について
NACRES:
NA.26
UNSPSC Code:
12352200
Specific activity:
≥ 50 units/mg protein
Assay:
≥70%
Biological source:
Bordetella pertussis Tohama I
assay
≥70%
form
liquid
biological source
Bordetella pertussis Tohama I
specific activity
≥ 50 units/mg protein
technique(s)
cell culture | mammalian: suitable
suitability
suitable for molecular biology
application(s)
detection
storage temp.
−20°C
Quality Level
Gene Information
Bordetella pertussis Tohama I ... CyaA(69600712)
General description
Research area: IMMUNO AND CKS
Adenylate Cyclase Toxin (ACT or CyaA) is a member of the extensive family of toxins known as Repeat in Toxin (RTX), which are produced by Gram-negative organisms. ACT is encoded by the cyaA gene and secreted extracellularly in the form of a soluble protein. It exhibits both adenylate cyclase enzymatic activity and hemolytic activity. The synthesis, maturation, and secretion of ACT are regulated by the CyaCABD operon. Moreover, its specific cellular receptor, CD11b/CD18 integrin (αMβ2, Mac-1, or CR3), is expressed on myeloid phagocytes.
Adenylate Cyclase Toxin (ACT or CyaA) is a member of the extensive family of toxins known as Repeat in Toxin (RTX), which are produced by Gram-negative organisms. ACT is encoded by the cyaA gene and secreted extracellularly in the form of a soluble protein. It exhibits both adenylate cyclase enzymatic activity and hemolytic activity. The synthesis, maturation, and secretion of ACT are regulated by the CyaCABD operon. Moreover, its specific cellular receptor, CD11b/CD18 integrin (αMβ2, Mac-1, or CR3), is expressed on myeloid phagocytes.
Application
Adenylyl Cyclase Toxin from Bordetella pertussis has been used as Gα(i/o) inhibitor to study the involvement of the sphingosine 1-phosphate receptor 2/Gα(12/13)/MAPK signaling pathway in the priming and activation of NLRP3 inflammasome during cholestatic liver injury.
Biochem/physiol Actions
Adenylate Cyclase Toxin (CyaA) is responsible for inhibiting the phagocytic activities of neutrophils and macrophages by impairing oxidative response and chemotaxis, ultimately leading to cell apoptosis or necrosis. Additionally, ACT can upregulate the expression of MHC class II and costimulatory molecules on dendritic cells, thereby reducing proinflammatory cytokine production.
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
SAE0066-50UG: + SAE0066-50UG-PW: + SAE0066-BULK: + SAE0066-VAR:
jan
Pertussis toxin and adenylate cyclase toxin: key virulence factors of Bordetella pertussis and cell biology tools
Carbonetti NH
Future Microbiology, 5, 455?469-455?469 (2010)
Understanding the Mechanism of Translocation of Adenylate Cyclase Toxin across Biological Membranes
Ostolaza H, et al.
Toxins, 9(10), 295-295 (2017)
Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy
Chenal A and Ladant D
Toxins, 10(7), 302-302 (2018)
NLRP3 inflammasome priming and activation in cholestatic liver injury via the sphingosine 1-phosphate/S1P receptor 2/G?(12/13)/MAPK signaling pathway
Hou L, et al.
Journal of Molecular Medicine, 99, 273?288-273?288 (2021)
Lei Hou et al.
Journal of molecular medicine (Berlin, Germany), 99(2), 273-288 (2021-01-04)
NLRP3 inflammasome-driven inflammation represents a key trigger for hepatic fibrogenesis during cholestatic liver injury. However, whether sphingosine 1-phosphate (S1P) plays a role in NLRP3 inflammasome priming and activation remains unknown. Here, we found that the expression of NLRP3 in macrophages
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）