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Merck

T3320

Anti-γ-Tubulin Antibody

rabbit polyclonal

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00677366
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, ICC, WB
Species reactivity:
human, frog, rat
Citations:
62
Technique(s):
immunocytochemistry: 1:1,000 using methanol-acetone fixed, cultured rat, RAT-2 cells, microarray: suitable, western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line., western blot: 1:5,000 using Xenopus eggs extract
Uniprot accession no.:
P23258

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製品名

抗γ-チューブリン (AK-15) ウサギ宿主抗体, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

human, frog, rat

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: 1:1,000 using methanol-acetone fixed, cultured rat, RAT-2 cells, microarray: suitable, western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line., western blot: 1:5,000 using Xenopus eggs extract

UniProt accession no.

P23258

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBG1(7283), TUBG2(27175)
rat ... Tubg1(252921), Tubg2(680991)

関連するカテゴリー

Physical form

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

Application

Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in following studies:
  • Western blot.
  • Immunofluorescence.
Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in:
  • western blotting
  • immunocytochemistry (immunofluorescence staining
  • immunofluorescence

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression ofγ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

γ-Tubulin (48 kDa) is a highly conserved protein. The microtubule cytoskeleton consists of a dynamic, highly polarized network of microtubules filaments, microtubule-associated proteins, microtubule motors, and microtubule-organizing proteins. γ-Tubulin is found as part of a large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule.
γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ -Tubulin consists of two isoforms tubulinγ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ -tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

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保管分類

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

IXO11329: + IXO11107: + T3320-.2ML: + T3320-VAR: + T3320-BULK: + IXO11469:

jan

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試験成績書（COA）

Lot/Batch Number
0000515685
0000515685
0000439642
0000439642
0000216731

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以前この製品を購入いただいたことがある場合

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文書ライブラリにアクセスする

l-Amino acid oxidase isolated from Calloselasma rhodostoma snake venom induces cytotoxicity and apoptosis in JAK2V617F-positive cell lines
Tavares C, et al.
Revista Brasileira de Hematologia e Hemoterapia, 38(2), 128-134 (2016)
L-amino acid oxidase isolated from Bothrops pirajai induces apoptosis in BCR-ABL-positive cells and potentiates imatinib mesylate effect.
Burin SM
Basic and Clinical Pharmacology and Toxicology, 113(2), 103-112 (2013)
L-Amino Acid Oxidase Isolated from B othrops pirajai Induces Apoptosis in BCR-ABL-Positive Cells and Potentiates Imatinib Mesylate Effect
Burin SM, et al.
Basic and Clinical Pharmacology and Toxicology, 113, 103-112 (2013)
Cristiane Tavares et al.
Revista brasileira de hematologia e hemoterapia, 38(2), 128-134 (2016-05-22)
Myeloproliferative neoplasms are Philadelphia chromosome-negative diseases characterized by hyperproliferation of mature myeloid cells, associated or not with the Janus kinase 2 tyrosine kinase mutation, JAK2V617F. As there is no curative therapy, researchers have been investigating new drugs to treat myeloproliferative
The structure of the gamma-tubulin small complex: implications of its architecture and flexibility for microtubule nucleation
Kollman JM, et al.
Molecular Biology of the Cell, 19(1), 207-215 (2008)
関連する文献をすべて表示

資料

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

εチューブリンを含むチューブリンに対する抗体

αチューブリンとβチューブリンのヘテロダイマーで構成される真核細胞の細胞骨格の微小管には、多様な機能を発揮する複数の明確なチューブリンクラスがあります。

関連コンテンツ

Data Sheet - T3320

Instructions

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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