T3411
テトラメチルアンモニウムクロリド 溶液
Molecular Biology
別名:
N,N,N-Trimethylmethanaminium chloride
InChI
1S/C4H12N.ClH/c1-5(2,3)4;/h1-4H3;1H/q+1;/p-1
SMILES string
[Cl-].C[N+](C)(C)C
InChI key
OKIZCWYLBDKLSU-UHFFFAOYSA-M
grade
Molecular Biology
concentration
5 M
foreign activity
DNase, RNase, none detected
Quality Level
関連するカテゴリー
General description
テトラメチルアンモニウムは、AT-リッチのDNAポリマーに結合すると同時に、GC塩基対よりも優先的なAT塩基対の融解を消失させます。18 ㏁水の0.2 μm フィルターろ過済み溶液でお届けします。
Application
テトラメチルアンモニウムクロリド溶液（TMAC）は、次の用途で使用されています：
- アレイハイブリダイゼーションとスキャニング用のハイブリダイゼーションカクテルの調製
- 次世代シーケンシング（NGS）、ならびにシーケンシングによるゲノムワイドな偏りのない二本鎖切断の同定（GUIDE-seq）のライブラリー調製
- ハイブリダイゼーションと検出のためのTMACバッファーおよびビーズハイブリダイゼーションミックスの調製
W B Melchior et al.
Proceedings of the National Academy of Sciences of the United States of America, 70(2), 298-302 (1973-02-01)
Several small alkylammonium ions can eliminate, or even reverse, the usual dependence of the DNA transition temperature on base composition. For example, in 3 M tetramethylammonium chloride, or 2.4 M tetraethylammonium chloride, DNAs of different base compositions all melt at
Nikolay L Malinin et al.
Nature protocols, 16(12), 5592-5615 (2021-11-14)
Genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) is a sensitive, unbiased, genome-wide method for defining the activity of genome-editing nucleases in living cells. GUIDE-seq is based on the principle of efficient integration of an end-protected double-stranded oligodeoxynucleotide
Hybridization of genomic DNA to oligonucleotide probes in the presence of tetramethylammonium chloride.
A G DiLella et al.
Methods in enzymology, 152, 447-451 (1987-01-01)
Yanwen Guo et al.
Methods in molecular biology (Clifton, N.J.), 1176, 33-44 (2014-07-18)
As small noncoding RNAs, microRNAs (miRNAs) regulate diverse biological functions, including physiological and pathological processes. The expression and deregulation of miRNA levels contain rich information with diagnostic and prognostic relevance and can reflect pharmacological responses. The increasing interest in miRNA-related
Xiao-Yong Li et al.
Methods in molecular biology (Clifton, N.J.), 809, 3-26 (2011-11-25)
Immunoprecipitation of cross-linked chromatin in combination with microarrays (ChIP-chip) or ultra high-throughput sequencing (ChIP-seq) is widely used to map genome-wide in vivo transcription factor binding. Both methods employ initial steps of in vivo cross-linking, chromatin isolation, DNA fragmentation, and immunoprecipitation.
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