T9784
トリシン
BioXtra, pH 4.0-6.0 (1 M in H2O), ≥99% (titration)
別名:
N-[トリス(ヒドロキシメチル)メチル]グリシン
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この商品について
化学式:
(HOCH2)3CNHCH2CO2H
CAS番号:
分子量:
179.17
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
227-193-6
Beilstein/REAXYS Number:
1937804
MDL number:
product line
BioXtra
Quality Level
assay
≥99% (titration)
form
powder
impurities
≤0.005% Phosphorus (P), ≤0.1% Insoluble matter
ign. residue
≤0.1%
pH
4.0-6.0 (1 M in H2O)
useful pH range
7.4-8.8
pKa (25 °C)
8.1
solubility
H2O: 1 M, clear, colorless
anion traces
chloride (Cl-): ≤0.1%, sulfate (SO42-): ≤0.05%
cation traces
Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%
absorption
≤0.05 at 280 in H2O at 1 M, ≤0.1 at 260 in H2O at 1 M
application(s)
diagnostic assay manufacturing
SMILES string
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
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General description
The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.
Application
低分子量ペプチド分離用のバッファー成分です。
Tricine has been used:
- in a polymerase chain reaction (PCR) buffer used in the single-cell collection
- as a component of lactotroph feeding medium for culturing lactotrophs
- to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer
保管分類
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
関連コンテンツ
Product Information Sheet
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