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Merck

T9822

モノクロナール抗チューブリン, ポリグルタミル化 マウス宿主抗体

clone B3, purified from hybridoma cell culture

別名:

Anti-K-ALPHA-1

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03792435
Conjugate:
unconjugated
Clone:
B3, monoclonal
Application:
ARR, ELISA (i), ICC, WB
Citations:
41

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B3, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract

isotype

IgM

UniProt accession no.

P68363

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBA4A(7277), TUBB(203068)
mouse ... Tuba1a(22142), Tubb2b(73710)
rat ... Tuba1a(64158)

関連するカテゴリー

General description

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).
Tubulin is a cellular colchicine-binding protein.

Immunogen

精製ウニLytechinus pictus精子軸糸タンパク質。

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:
  • immunoprecipitation
  • immunohistochemistry
  • immunofluorescence

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Biochem/physiol Actions

Microtubule plays an important role in DNA segregation.

Physical form

0.01M PBS溶液(pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

T9822-BULK: + T9822-VAR: + T9822-.2ML: + T9822-100UL:

jan

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試験成績書（COA）

Lot/Batch Number
0000453505
0000453505
0000371016
0000307927
0000307926

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Jenny Yamauchi et al.
Human molecular genetics, 22(21), 4306-4317 (2013-06-19)
Merosin-deficient congenital muscular dystrophy type 1A (MDC1A) is an autosomal recessive disorder caused by mutations in the laminin-α2 gene (OMIM: 607855). Currently, no treatment other than palliative care exists for this disease. In our previous work, genetic interventions in the
Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain
Ravelli RBG, et al.
Nature, 428(6979), 198-198 (2004)
SDCCAG8 interacts with RAB effector proteins RABEP2 and ERC1 and is required for hedgehog signaling
Airik R, et al.
PLoS ONE, 11(5), e0156081-e0156081 (2016)
Olga V Plotnikova et al.
Methods in cell biology, 94, 137-160 (2009-01-01)
Cilia are microtubule-based structures that protrude from the cell surface and function as sensors for mechanical and chemical environmental cues that regulate cellular differentiation or division. In metazoans, ciliary signaling is important during organismal development and in the homeostasis controls
Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics
Kline-Smith SL and Walczak CE
Molecular Cell, 15(3), 317-327 (2004)
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資料

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

εチューブリンを含むチューブリンに対する抗体

αチューブリンとβチューブリンのヘテロダイマーで構成される真核細胞の細胞骨格の微小管には、多様な機能を発揮する複数の明確なチューブリンクラスがあります。

関連コンテンツ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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