コンテンツへスキップ
Merck

T9822

モノクロナール抗チューブリン, ポリグルタミル化 マウス宿主抗体

clone B3, purified from hybridoma cell culture

別名:

Anti-K-ALPHA-1

ログインで組織・契約価格をご覧ください。

サイズを選択してください

この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03792435

主要文書

すべての文書を表示
テクニカルサービス
お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。
お手伝いします
テクニカルサービス
お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。
お手伝いします

製品名

モノクロナール抗チューブリン, ポリグルタミル化 マウス宿主抗体, clone B3, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B3, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract

isotype

IgM

UniProt accession no.

P68363

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBA4A(7277), TUBB(203068)
mouse ... Tuba1a(22142), Tubb2b(73710)
rat ... Tuba1a(64158)

関連するカテゴリー

Immunogen

精製ウニLytechinus pictus精子軸糸タンパク質。

Physical form

0.01M PBS溶液(pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:
  • immunoprecipitation
  • immunohistochemistry
  • immunofluorescence

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Biochem/physiol Actions

Microtubule plays an important role in DNA segregation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).
Tubulin is a cellular colchicine-binding protein.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください

保管分類

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
0000453505
0000453505
0000371016
0000307927
0000307926

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

COAを検索

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Jenny Yamauchi et al.
Human molecular genetics, 22(21), 4306-4317 (2013-06-19)
Merosin-deficient congenital muscular dystrophy type 1A (MDC1A) is an autosomal recessive disorder caused by mutations in the laminin-α2 gene (OMIM: 607855). Currently, no treatment other than palliative care exists for this disease. In our previous work, genetic interventions in the
SDCCAG8 interacts with RAB effector proteins RABEP2 and ERC1 and is required for hedgehog signaling
Airik R, et al.
PLoS ONE, 11(5), e0156081-e0156081 (2016)
Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain
Ravelli RBG, et al.
Nature, 428(6979), 198-198 (2004)
A Mutation in CCDC50, a Gene Encoding an Effector of Epidermal Growth Factor-Mediated Cell Signaling, Causes Progressive Hearing Loss
Modamio-HoybjorS, et al.
American Journal of Human Genetics, 80(6), 1076-1089 (2007)
Karel Soucek et al.
The Prostate, 66(9), 954-965 (2006-03-17)
Multiple diverse posttranslational modifications of alpha-tubulin such as detyrosination, further cleavage of the penultimate glutamate residue (Delta2-tubulin), acetylation, and polyglutamylation increase the structural and functional diversity of microtubules. Herein, we characterized the molecular profile of alpha-tubulin posttranslational modifications in normal
関連する文献をすべて表示

資料

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

εチューブリンを含むチューブリンに対する抗体

αチューブリンとβチューブリンのヘテロダイマーで構成される真核細胞の細胞骨格の微小管には、多様な機能を発揮する複数の明確なチューブリンクラスがあります。

関連コンテンツ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら（テクニカルサービス）