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この商品について
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
73
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mammals
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates), indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells, western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates
shipped in
dry ice
storage temp.
−20°C
General description
The Anti-VSV-G rabbit antibody reacts with amino- and carboxyl-terminal VSV-G tagged fusion proteins.
Vesicular stomatitis viruses (VSV) belongs to the Vesiculovirus genus of the Rhabdoviridae family. Vesicular stomatitis virus G (VSV G) protein is considered to be the early recognized type III viral fusion protein.
Immunogen
水疱性口内炎ウイルス糖タンパク質(VSV-G)のアミノ酸残基497~511番(N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C)に相当する合成ペプチドであり、C末端に付加したシステイン残基を介してマレイミド活性化KLHに結合しています。抗体はVSV-GタグのC末端とN末端の両方を認識します。
Application
Antibody is suitable for immunoblotting, immunoprecipitation, and indirect immunofluorescence.
immunocytochemistry.
immunocytochemistry.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Western Blotting (1 paper)
Western blotting following immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Western Blotting (1 paper)
Western blotting following immunoprecipitation (1 paper)
Anti-VSV-G antibody produced in rabbit has been used in western blotting and immunoprecipitation.
Biochem/physiol Actions
Vesicular stomatitis virus G (VSV G) is capable of stimulating cell-cell fusion upon low pH stimulation. The transmembrane region of VSV G protein is highly essential for the virus transition from the hemi-fusion to full fusion state.
Physical form
0.01M PBS溶液(pH 7.4, 1%BSA, 防腐剤として15mMアジ化ナトリウム含有)
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保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
A mechanism for Wnt coreceptor activation
Tamai K, et al.
Molecular Cell, 13(1), 149-156 (2004)
Vesicular stomatitis virus glycoprotein is a determinant of pathogenesis in swine, a natural host
Martinez I, et al.
Journal of virology, 77(14), 8039-8047 (2003)
Vesicular stomatitis virus G protein transmembrane region is crucial for the hemi-fusion to full fusion transition
Ci Y, et al.
Scientific Reports, 8(1), 10669-10669 (2018)
Periodic fever with aphthous stomatitis, pharyngitis, and cervical adenitis syndrome is associated with a CARD8 variant unable to bind the NLRP3 inflammasome
Cheung MS, et al.
Journal of immunology (Baltimore, Md. : 1950), 198(5), 2063-2069 (2017)
p53-regulated networks of protein, mRNA, miRNA, and lncRNA expression revealed by integrated pulsed stable isotope labeling with amino acids in cell culture (pSILAC) and next generation sequencing (NGS) analyses
Hunten S, et al.
Molecular and Cellular Proteomics, 14(10), 2609-2629 (2015)
関連コンテンツ
Instructions
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