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Merck

S1797

SOC Medium

For use in transformation

동의어(들):

Super Optimal broth

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.85
UNSPSC Code:
41106200

주요 문서

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기술 서비스
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도움 문의
기술 서비스
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도움 문의

grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO4, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli, nonselective for coliforms

Quality Level

500

관련 카테고리

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Features and Benefits

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000498782
0000498784
0000498784
0000498782
0000434973

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted
Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers
Iqbal A
PLoS ONE (2015)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Qiao-Yang Sun et al.
Analytical biochemistry, 394(1), 144-146 (2009-07-23)
In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.
모든 관련된 서류 보기

문서

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

프로토콜

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

관련 콘텐츠

mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

mRNA 합성

mRNA 기반 백신 및 치료제 개발에서 플라스미드 준비, 시험관 내 전사, mRNA 정제 및 LNP 제형을 위한 도구입니다.

Directional Cloning System

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.