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Merck

WGA3

GenomePlex® WGA Reamplification Kit

Reamplification of WGA product with minimal bias

동의어(들):

GenomePlex® whole genome amplification reamplification kit, Whole genome reamplification kit

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.55
UNSPSC Code:
41121800

주요 문서

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기술 서비스
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도움 문의
기술 서비스
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도움 문의

Quality Level

200

technique(s)

whole genome amplification: suitable

dilution

(WGA)

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

관련 카테고리

General description

GenomePlex® WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It allows the user to obtain even higher yields of desired DNA without disturbing the original, limited source of genomic DNA. The genomic DNA can be reamplified up to five cycles with minimal allele dropout producing an even higher yield. It provides the researcher to generate a representative, ~500-fold amplification of genomic DNA.

Application

GenomePlex® WGA Reamplification Kit has been used to generate DNA probes (μBm-probe and μBsm-probe) labeled with digoxigenin-11-dUTP. It has also been used in the reamplification of WGA2-amplified genomic DNA to generate >10 μg gDNA for microarray hybridization.

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.

키트 구성품 전용

제품 번호
설명
  • Deoxynucleotide Mix, 10 mM

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .2 mLSDS

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000473462
0000473462
0000441854
0000374640
SLCN5650

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Mirella L Meyer-Ficca et al.
Chromosoma, 122(4), 319-335 (2013-06-05)
The mammalian sperm nucleus is characterized by unique properties that are important for fertilization. Sperm DNA retains only small numbers of histones in distinct positions, and the majority of the genome is protamine associated, which allows for extreme condensation and
Kira S Zadesenets et al.
International journal of molecular sciences, 21(2) (2020-01-24)
The genus Macrostomum represents a diverse group of rhabditophoran flatworms with >200 species occurring around the world. Earlier we uncovered karyotype instability linked to hidden polyploidy in both M. lignano (2n = 8) and its sibling species M. janickei (2n
Yun Xia et al.
Frontiers in genetics, 10, 1364-1364 (2020-02-11)
Chromosomal rearrangements have long fascinated evolutionary biologists for being widely implicated in causing genetic differentiation. Suppressed recombination has been demonstrated in various species with inversion; however, there is controversy over whether such recombination suppression would facilitate divergence in reciprocal translocation
Churamani Khanal et al.
Plant disease, 103(8), 1835-1842 (2019-06-14)
This study employed single nucleotide polymorphisms (SNPs) to determine the genetic variability present in 26 isolates of Rotylenchulus reniformis from Louisiana, Mississippi, Arkansas, South Carolina, Georgia, Hawaii, and Alabama. Genomic DNA from reniform nematode was extracted and increased quantitatively using
Elizabeth P Murchison et al.
Cell, 148(4), 780-791 (2012-02-22)
The Tasmanian devil (Sarcophilus harrisii), the largest marsupial carnivore, is endangered due to a transmissible facial cancer spread by direct transfer of living cancer cells through biting. Here we describe the sequencing, assembly, and annotation of the Tasmanian devil genome
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