Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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The field of Oncology is largely concerned with elucidating how cancers develop and how best to develop therapies to treat them. Oncologists commonly employ microscopy not only to identify and classify cancer cells, but also to identify the fragmented nuclei of apoptotic cells treated with a chemotherapeutic drug, or to visualize the binding and subsequent internalization and trafficking of therapeutic monoclonal antibodies. Amnis® imaging flow cytometry is ideally suited to these types of studies, and gives the added benefit of combining immunophenotyping with quantitative morphometric measurements that can be applied to identifying rare sub-populations of potentially metastatic cells, including circulating tumor cells (CTC).
Co-Localization of an Antibody-Drug Conjugate to Endosomes or Lysosomes
In this experiment we use the capabilities of the Imagestream®x system to measure the transit of a fluorescently labeled antibody-drug conjugate (ADC) through the cellular endocytic pathway. The high spatial resolution afforded by the Imagestream®x system allowed accurate measurement of ADC co-localization to endosomes and lysosomes. See the application note for more details.
Apoptotic Index Using the ImageStream
DNA condensation and fragmentation are hallmarks of apoptosis. By measuring the area and the intensities of the brightest portions of the nuclear image, the bright, punctate nuclear imagery of apoptotic cells can be distinguished from the evenly stained nuclear imagery of a normal, healthy nucleus. This makes possible the automated identification of apoptotic cells. When cells begin to dye by apoptosis there is fragmentation and condensation of the DNA. By measuring the area and the intensities of the brightest portions of the nuclear image, the bright, punctate nuclear imagery of apoptotic cells can be distinguished from the evenly stained nuclear imagery of a normal, healthy nucleus. This makes possible the automated identification of apoptotic nuclear morphology. See the application note for more details.
The field of Oncology is largely concerned with elucidating how cancers develop and how best to develop therapies to treat them. Oncologists commonly employ microscopy not only to identify and classify cancer cells, but also to identify the fragmented nuclei of apoptotic cells treated with a chemotherapeutic drug, or to visualize the binding and subsequent internalization and trafficking of therapeutic monoclonal antibodies. Amnis® imaging flow cytometry is ideally suited to these types of studies, and gives the added benefit of combining immunophenotyping with quantitative morphometric measurements that can be applied to identifying rare sub-populations of potentially metastatic cells, including circulating tumor cells (CTC).
