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HCYP2MAG-62K MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay


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Replacement Information

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Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
6Ckine, BCA-1, CTACK, ENA-78, Eotaxin-2, Eotaxin-3, I-309, IL-16, IL-20, IL-21, IL-23, IL-28A, IL-33, LIF, MCP-2, MCP-4, MIP-1d, SCF, SDF-1A+β, TARC, TPO, TRAIL, TSLPHMplexLuminex xMAP
Catalogue NumberHCYP2MAG-62K
Trade Name
DescriptionMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing

Therefore, the MILLIPLEX® MAP Human Cytokine / Chemokine Panel II enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

PLoS One. 2016 Feb 26;11(2):e0149411. doi: 10.1371/journal.pone.0149411. eCollection 2016.
B Cell, Th17, and Neutrophil Related Cerebrospinal Fluid Cytokine/Chemokines Are Elevated in MOG Antibody Associated Demyelination.
Kothur K, Wienholt L, Tantsis EM, Earl J, Bandodkar S, Prelog K, Tea F, Ramanathan S, Brilot F, Dale RC
Background Information“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: 4 - 9
  • Inter-assay: 6 - 17
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.

• This assay requires 25 µl of neat plasma or serum, or 25 µL cell culture supernatant per well.

• This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • 6Ckine
  • BCA-1
  • ENA-78
  • Eotaxin-2
  • Eotaxin-3
  • I-309
  • IL-16
  • IL-20
  • IL-21
  • IL-23
  • IL-28A
  • IL-33
  • LIF
  • MCP-2
  • MCP-4
  • MIP-1d
  • SCF
  • SDF-1A+β
  • TARC
  • TPO
  • TSLP
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies and any of the other analytes in this panel.
  • 81% - 110%
Physicochemical Information
  • 0.4 - 55.8 pg/mL
Linearity of DilutionContact Technical Service for this value
Standard Curve Range
  • 1.0 – 100,000 pg/mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information



Protocol: Human Cytokine / Chemokine Panel II

MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay SDS


Safety Data Sheet (SDS) 


Reference overviewPub Med ID
Evidence of enhanced systemic inflammation in stable kidney transplant recipients with low Framingham risk scores.
Mansell, H; Rosaasen, N; Dean, J; Shoker, A
Clinical transplantation  27  E391-9  2013

Show Abstract
23782452 23782452
IL-12p70-producing patient DC vaccine elicits Tc1-polarized immunity.
Carreno, BM; Becker-Hapak, M; Huang, A; Chan, M; Alyasiry, A; Lie, WR; Aft, RL; Cornelius, LA; Trinkaus, KM; Linette, GP
The Journal of clinical investigation  123  3383-94  2013

Show Abstract
23867552 23867552


A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services
MAGPIX® and Luminex 200¿ Instruments Provide Equivalent Multiplex Detection of Inflammation Pathway Analytes Application Note (MM)
The Power of Biomarker Analysis

Technical Info

96 well Template 2 Up
96 well Template Single
HCYP2MAG-62K xPonent Protocol Template Version Magpix
HCYP2MAG-62K xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex Human Cytokine/Chemokine Panel II; Milliplex Human Cytokine/Chemokine Magnetic Panel II

Data Sheet

Full Service Custom-built Assays (Merck)


MILLIPLEX MAP: The Immunology of Cancer

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