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HCYP3MAG-63K MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel III - Immunology Multiplex Assay

HCYP3MAG-63K
Design And Price Your Kit

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
GCP2, HCC-1, I-TAC, IL-11, IL-29, Lymphotactin, M-CSF, MIG, MIP-3α/CCL20, MIP-3β, NAP2HMplexPlease note: Sample Type must be selected when configuring this kit. HCC-1/CCL14a and NAP-2/CXCL7 cannot be plexed with other analytes for serum/plasma.Luminex xMAP
Description
Catalogue NumberHCYP3MAG-63K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel III - Immunology Multiplex Assay
OverviewReferences:
PLoS One. 2016 Feb 26;11(2):e0149411. doi: 10.1371/journal.pone.0149411. eCollection 2016.
B Cell, Th17, and Neutrophil Related Cerebrospinal Fluid Cytokine/Chemokines Are Elevated in MOG Antibody Associated Demyelination.
Kothur K, Wienholt L, Tantsis EM, Earl J, Bandodkar S, Prelog K, Tea F, Ramanathan S, Brilot F, Dale RC
Background InformationMILLIPLEX MAP Human Cytokine/Chemokine Panel III enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: 2 - 9
  • Inter-assay: 4 - 24
Multiplex Ordering RequirementsPlease note: Sample Type must be selected when configuring this kit. HCC-1/CCL14a and NAP-2/CXCL7 cannot be plexed with other analytes for serum/plasma.
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• This assay requires 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• A 1:100 dilution is required for NAP2/CXCL7 and HCC-1/CCL14a for serum and plasma samples.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • GCP2
  • HCC-1
  • I-TAC
  • IL-11
  • IL-29
  • Lymphotactin
  • M-CSF
  • MIG
  • MIP-3α/CCL20
  • MIP-3β
  • NAP2
Assay PanelEverything you need in a single kit.
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 92%–106%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual cytokines/chemokines.
Standard Curve Range
  • 2.0 – 200,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human Cytokine /Chemokine Magnetic Bead Panel III

MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel III - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
MAGPIX® and Luminex 200¿ Instruments Provide Equivalent Multiplex Detection of Inflammation Pathway Analytes Application Note (MM)
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HCYP3MAG-63K xPonent Protocol Template Version Magpix
HCYP3MAG-63K xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex Human Cytokine/Chemokine Panel III

Data Sheet

Title
Full Service Custom-built Assays (Merck)

Posters

Title
MILLIPLEX MAP: The Immunology of Cancer
MILLIPLEX® MAP Cell Signaling Wall Chart (MM)

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Categories

Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits
Life Science Research > Antibodies and Assays > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits