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71003 NovaTaq™ DNA Polymerase

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71003
  
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      Overview

      Replacement Information
      Description
      OverviewNovaTaq™ DNA Polymerase* is a premium quality, recombinant form of Thermus aquaticus DNA polymerase that is licensed for PCR. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays. NovaTaq DNA Polymerase has an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl2, and 25 mM MgCl2 to enable convenient optimization of Mg2+ concentration.
      Source Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
      Concentration 5.0 U/µl
      Purity > 95% homogeneous by SDS-PAGE
      Endonuclease None detected
      Amplification effiency Functional PCR
      Storage –20°C

      Catalogue Number71003 Brand Family Novagen®

      References
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 74°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-<Sup>32</Sup>P]dCTP, and activated salmon sperm DNA.
      100 U, 500 U, or 5 × 500 UNovaTaq DNA Polymerase
      1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer with MgCl₂
      1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer without MgCl₂
      1 or 2 or 7 × 1.5 ml25 mM MgCl₂
      DeclarationPurchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71003 0

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      Categories

      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > PCR & RT-PCR Kits & Reagents