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521215 PDGF, AA Homodimer, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Pre-B Cell Stimulation Factor/Stromal Cell-Derived Factor-βrhPDGF-AA

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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      OverviewRecombinant, human platelet derived growth factor AA (disulfide-linked dimer of two A-chain monomers) expressed in E. coli. Amino acid sequence is the long form of mature human PDGF-A chain and is identical to that of the native human nucleotide sequence except for the addition of an N-terminal methionine group. Implicated in the differentiation of cells of the oligodendrocyte lineage.
      Catalogue Number521215
      Brand Family Calbiochem®
      SynonymsPre-B Cell Stimulation Factor/Stromal Cell-Derived Factor-βrhPDGF-AA
      References
      ReferencesButt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
      Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
      Betsholtz, C., et al. 1986. Nature 320, 695.
      Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
      Product Information
      FormLyophilized
      FormulationLyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activity1-3 ng/ml
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 EU/µg PDGF-AA
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      521215 0

      Documentation

      PDGF, AA Homodimer, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      PDGF, AA Homodimer, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      521215

      References

      Reference overview
      Butt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
      Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
      Betsholtz, C., et al. 1986. Nature 320, 695.
      Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-May-2008 RFH
      SynonymsPre-B Cell Stimulation Factor/Stromal Cell-Derived Factor-βrhPDGF-AA
      DescriptionRecombinant, human platelet derived growth factor AA (disulfide-linked dimer of two A-chain monomers) expressed in E. coli. Amino acid sequence is the long form of mature human PDGF-A chain and is identical to that deduced from the native human nucleotide sequence except for the addition of an N-terminal methionine group.
      FormLyophilized
      FormulationLyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 EU/µg PDGF-AA
      Biological activity1-3 ng/ml
      SolubilityReconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C.
      Toxicity Standard Handling
      Merck USA index14, 7525
      ReferencesButt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
      Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
      Betsholtz, C., et al. 1986. Nature 320, 695.
      Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors