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521200 PDGF, Human Platelets

PDGF, Human Platelets MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: nhPDGF

521200
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.





      Native platelet-derived growth factor from human platelets. Consists of approximately 70% PDGF-AB and 30% PDGF-BB. PDGF action is mediated by cell surface α- and β-receptors. M.W. 28,000-32,000.
      Catalogue Number521200
      Brand Family Calbiochem®
      SynonymsnhPDGF
      References
      ReferencesAbboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
      Soma, Y., et al. 1994. Exp. Cell Res. 212, 274.
      Hammacher, A., et al. 1988. J. Biol. Chem. 263, 16493.
      Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered 25% acetonitrile, 0.1% TFA.
      Applications
      Biological Information
      Biological activityED₅₀ = 1-3 ng/ml as measured in a mitogenic assay using quiescent NR 6-3T3 fibroblasts
      Purity≥97% by SDS-PAGE
      SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 EU/µg PDGF
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      521200 0

      Documentation

      References

      Reference overview
      Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
      Soma, Y., et al. 1994. Exp. Cell Res. 212, 274.
      Hammacher, A., et al. 1988. J. Biol. Chem. 263, 16493.
      Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-May-2008 RFH
      SynonymsnhPDGF
      DescriptionNative platelet-derived growth factor from human platelets. Consists of approximately 70% PDGF-AB and may contain about 30% PDGF-BB. PDGF action is mediated by cell surface α- and β-receptors.
      FormLyophilized
      FormulationLyophilized from sterile-filtered 25% acetonitrile, 0.1% TFA.
      SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 EU/µg PDGF
      Biological activityED₅₀ = 1-3 ng/ml as measured in a mitogenic assay using quiescent NR 6-3T3 fibroblasts
      SolubilityReconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Toxicity Standard Handling
      Merck USA index14, 7525
      ReferencesAbboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
      Soma, Y., et al. 1994. Exp. Cell Res. 212, 274.
      Hammacher, A., et al. 1988. J. Biol. Chem. 263, 16493.
      Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors