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CP70
Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)
liquid, clone FBN11, Calbiochem®
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
FBN11, monoclonal
Formulario
liquid
contiene
≤0.1% sodium azide as preservative
reactividad de especies
rat, human, mouse
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
do not freeze
dilution
(Frozen Sections (0.5-1 µg/mL)
Immunoblotting (0.5-1 µg/mL)
Immunofluorescence
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/mL, heat pre-treatment required))
isotipo
IgG1
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... FN1(2335)
mouse ... Fn1(14268)
rat ... Fn1(25661)
Categorías relacionadas
Descripción general
Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.
Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.
Inmunógeno
recombinant human fibronectin
Aplicación
Frozen Sections (0.5-1 µg/ml)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
Envase
Please refer to vial label for lot-specific concentration.
Nota de análisis
Positive Control
SW 156 cells or normal kidney tissue
SW 156 cells or normal kidney tissue
Otras notas
Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Toxicity: Standard Handling (A)
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Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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