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UNSPSC Code:
41116100
NACRES:
NA.54
Assay:
97.7% (HPLC)
Form:
solution
Solubility:
water: miscible
Storage temp.:
−20°C
Servicio técnico
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Permítanos ayudarleassay
97.7% (HPLC)
form
solution
mol wt
Mr 987.5 (biotin-16-UTP-Li4)
packaging
pkg of 25 μL (250 nmol; 10mM)
manufacturer/tradename
Roche
color
colorless
solubility
water: miscible
shipped in
dry ice
storage temp.
−20°C
General description
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Application
Biotin-labeled RNA can be used as a hybridization probe for:
- Northern blots
- Southern blots
- Plaque or colony lifts
- RNase protection experiments
- In situ hybridization
- Microarray hybridization
Physical form
Biotin-16-UTP, tetralithium salt, 10mM solution
Analysis Note
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Other Notes
For life science research only. Not for use in diagnostic procedures.
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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