A4414
Albumin from porcine serum
lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
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Número CAS:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
Porcine
Assay:
≥99% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
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Permítanos ayudarlebiological source
Porcine
assay
≥99% (agarose gel electrophoresis)
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
Porcine ... ALB(396960)
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General description
Albumin is present abundantly in serum. Porcine serum albumin (PSA) comprises a flexible C-terminal region compared to other albumins.
Application
Albumin from porcine serum has been used:
- as a non-hazardous protein to test its effect on T84 polarized intestinal monolayers and Caco2 polarized intestinal monolayers barrier integrity in trans-Epithelial Electrical Resistance (TEER) assay
- to test its effect on the human intestinal epithelial cell lines
- to test its interaction with the biodegradable ester-functionalized gemini surfactant 16-E2-16 using ultraviolet (UV)-visible and circular dichroism (CD) spectroscopy measurements
Biochem/physiol Actions
Albumin binds to many blood associated proteins. Porcine serum albumin (PSA) is regarded as a major allergen. PSA is used in the immunosensors, molecularly imprinted nanogels (MIP-NGs) and enzyme-linked immunosorbent assay (ELISA) protein-based detection for assessing contamination, especially in meat products.
Preparation Note
Prepared from albumin (A2764).
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Chehasan Cheubong et al.
Analytical chemistry, 92(9), 6401-6407 (2020-04-14)
Accurate, simple, and valuable analytical methods for detection of food contamination are rapidly expanding to evaluate the validity of food product quality because of ethnic considerations and food safety. Herein molecularly imprinted nanogels (MIP-NGs), capable of porcine serum albumin (PSA)
Bryan P Hurley et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 92, 75-87 (2016-04-10)
Human intestinal epithelial cell lines (T84, Caco-2, and HCT-8) grown on permeable Transwell™ filters serve as models of the gastrointestinal barrier. In this study, this in vitro model system was evaluated for effectiveness at distinguishing between hazardous and non-hazardous proteins. Indicators
An ester-functionalized cationic gemini surfactant mediated structural transitions of porcine serum albumin (PSA) via binding interaction
Akram M, et al.
Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 516, 245-253 (2017)
Sung-Sil Moon et al.
Korean journal for food science of animal resources, 34(3), 355-361 (2014-01-01)
This study was conducted to evaluate the changes in porcine serum albumin (PSA), a major allergen, which occur when raw pork ham is marinated with kiwi or pineapple juice, and/or when the ham is pan broiled at 300℃ for 4
C Zimmermann et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 115, 451-459 (2018-04-04)
Recent studies suggest that human derived intestinal epithelial cells (IECs) cultured as polarized monolayers on Transwell® filters may respond differently when exposed to hazardous and non-hazardous proteins. This experimental platform was based on apical exposure of IEC monolayers to test
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