CS0390
Mitochondria Staining Kit
1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)
Sinónimos:
JC-1 dye
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352207
Nombre del producto
Mitochondria Staining Kit, 1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)
usage
kit sufficient for 200 tests (of 1 mL cell suspensions)
kit sufficient for 40 tests (of 5 mL cell suspensions)
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
flow cytometry: suitable
protein staining: suitable
fluorescence
λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.
Features and Benefits
Kit offers:
- A fast, simple, and convenient method for staining and assaying mitochondria intactness
- Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
- Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
- The shift in fluorescence is clearly detectable in both green and red channels
- Suitable for adherent cells as well as cells in suspension
- Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
- The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry
General description
In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.
Clase de almacenamiento
10 - Combustible liquids
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Instructions
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