M9942
Anti-MAP2 Antibody
mouse monoclonal, HM-2
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Permítanos ayudarleNombre del producto
Monoclonal Anti-MAP2 antibody produced in mouse, clone HM-2, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HM-2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~280 kDa
species reactivity
rat, chicken, human, mouse, bovine, quail
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using rat brain extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)
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Application
Anti-MAP2 antibody has been used in immunofluorescence. It has also been used in immunohistochemistry.
May be used in various immunochemical techniques including immunoblotting, immunohistochemistry, and immunocytochemistry. Monoclonals antibodies to MAP2 are used to study the cytoskeleton structure in cells.
By immunoblotting, a working antibody concentration of 1-2 μg/ml is recommended using a rat brain extract.
By immunoblotting, a working antibody concentration of 1-2 μg/ml is recommended using a rat brain extract.
Biochem/physiol Actions
MAP2 (microtubule-associated protein 2) expression is monitored to predict the disease progression in glioma. MAP2 is critical in the formation of dendritic protrusions in astrocytes and oligodendrocytes. In eukaryotes, MAP2 is essential for spindle assembly and chromosome segregation.
Reacts with all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. Does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.
This antibody recognizes all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. It does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280 kDa (MAP2a and b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and neurons have reached their mature morphology.
MAP2 is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.
MAP2 is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.
Immunogen
rat brain microtubule-associated proteins (MAPs).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 C for up to one month. For extended storage, freeze in working aliquots at 20 C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Modulation of mGluR-dependent MAP1B translation and AMPA receptor endocytosis by microRNA miR-146a-5p.
Chen Y L, et al.
The Journal of Neuroscience, 33(21), 9013-9020 (2013)
Expression of EAAT2 in neurons and protoplasmic astrocytes during human cortical development
DeSilva T M, et al.
The Journal of Comparative Neurology, 520(17), 3912-3932 (2012)
Remarkable reduction of MAP2 in the brains of scrapie-infected rodents and human prion disease possibly correlated with the increase of calpain.
Guo Y, et al.
PLoS ONE, 7, e30163-e30163 (2012)
Lipolysaccharide-induced neuroinflammation is associated with Alzheimer-like amyloidogenic axonal pathology and dendritic degeneration in rats.
Deng X, et al.
Advances in Alzheimer's disease, 3(2), 78-78 (2014)
The MAP2/Tau family of microtubule-associated proteins.
Dehmelt L and Halpain S
Genome Biology, 6(1), 204-204 (2005)
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