Anti-Protein A antibody produced in rabbit

Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus.

The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.

Protein A from Staphylococcus aureus

Anti-Protein A antibody produced in rabbit has been used in immunoblotting.

Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification.

Lyophilized from 0.01M phosphate buffered saline, pH 7.2

Reconstitute with 2 ml deionized water.

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