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SCC218 BCH869 Human H3K27M Glioma Cell lines

BCH869 cell line has been used in the investigation of radiosensitization factors and tumorigenesis pathways, and is a highly clinically relevant model for this especially serious form of pediatric glioma.

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SCC218
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC218
      DescriptionBCH869 Human H3K27M Glioma Cell lines
      Background InformationPediatric high-grade glioma (pHGG) and diffuse intrinsic pontine glioma (DIPG) are among the most aggressive and recalcitrant cancers, with extremely low survival rates. Diffuse midline gliomas (DMG) with histone H3 lysine27-to-methionine mutations (H3K27M glioma) represent a highly aggressive subtype of glioma, which predominantly arise in children and young adults. The overall prognosis of H3K27M glioma is poor, displaying median survival rates of approximately 9 to 11 months.2 Aberrant activation of the PI3K pathway via PTEN silencing is a contributor to tumorigenesis in most pHG and DIP gliomas.
      The BCH869 H3K27M glioma cell line is a representative model for H3K27M diffuse glioma. BCH869 cells grow as neurospheres and carry mutations in oncogenes ACVR1, PIK3CA, and PPM1D in addition to H3K27M mutation.3 BCH869 cells express EGFR, a common glioma marker, and have been validated via staining for the H3.3 K27M oncohistone mutant. BCH869 cell line has been used in the investigation of radiosensitization factors and tumorigenesis pathways, and is a highly clinically relevant model for this especially serious form of pediatric glioma.

      Source
      BCH869 cell line was derived from Pons tissue of a 7-year-old female patient.4

      References
      1. Neuro Oncol 2017, 19(2):153-161.
      2. Front Oncol 2020, 9:1436.
      3. Cancer Res 2018, 78(14): 4007-4021.
      4. Suva et al. US Patent application pub. US 2020/0384022 A1
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationBCH869 cell line has been used in the investigation of radiosensitization factors and tumorigenesis pathways, and is a highly clinically relevant model for this especially serious form of pediatric glioma.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC218 04065266779974