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SCC246 YUMMER1G.1F Mouse Cell Line

The characteristics of the YUMMER1.G1F cell line make it a valuable model for investigating sex-related differences in immune checkpoint inhibition and mechanisms of anti-tumor responses.

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SCC246
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC246
      DescriptionYUMMER1G.1F Mouse Cell Line
      OverviewThe Yale University Mouse Melanoma (YUMM) lines are a comprehensive system of melanoma cell lines harboring defined genetic drivers of melanoma that are syngenic with the immunocompetent C57/B1/6 mouse model. YUMM cell lines were derived from the Braf/Pten genetically engineered mouse melanoma model, a highly penetrant GEMM in which melanomas develop with short latency. YUMM Exposed to Radiation (YUMMER) cell lines are clonal isolates of YUMM lines exposed to UVB radiation to reflect the high somatic mutation burden in melanomas. YUMMER cells harbor a high frequency of stable somatic mutations compared to YUMM cells; these mutations have been shown to stimulate host adaptive immune response.3 The YUMMER1.G1F mouse melanoma cell line is both immunocompetent and reflective of the somatic mutations common in melanomas. YUMMER1.G1F carries three driver mutations of melanoma: Braf V600E, Pten -/- and Cdkn2 -/-.1,2 YUMMER1.G1F cells are diploid, allowing for enhanced knockout frequency in CRISPR-based screens, are of female genotype, and have pheomelanin-producing background.4 The characteristics of the YUMMER1.G1F cell line make it a valuable model for investigating sex-related differences in immune checkpoint inhibition and mechanisms of anti-tumor responses.

      Source
      The YUMMER1.G1F mouse melanoma cell line is a diploid clonal isolate from YUMMER.G cells exposed to UVB radiation.4 The original YUMM1.1 cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a C57/B1/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing.1
      Background InformationThe great promise of immune-based therapies in cancer and recent progress in successful application of these approaches has brought to the fore the necessity of immune-competent models to evaluate immune system responses to cancer cells. Melanomas exhibit relatively high somatic mutation burden, and these mutations may act as neoantigens that generate anti-tumor immune responses. The development of immunocompetent cell models is critical to the advancement of cancer immunotherapy and understanding of immunity, although few tractable model systems are available.


      References
      1. Pigment Cell Melanoma Res 2016, 29(5): 590-597.
      2. Nat Genet 2009, 41(5): 544-552.
      3. Pigment Cell Melanoma Res 2017, 30(4): 428-435.
      4. Ramseier JY, Charos A, Park K, Damsky W, Bosenberg MW (2019). Cancer Res 79(13 Suppl) Abstract #4622.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationThe characteristics of the YUMMER1.G1F cell line make it a valuable model for investigating sex-related differences in immune checkpoint inhibition and mechanisms of anti-tumor responses.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC246 04065266920123