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SCC440 SK-MEL-147 Human Melanoma Cell Line 

The SK-MEL-147 cell line expresses PMEL and MelanA proteins, two common melanoma markers. PMEL has been implicated in poor immune cell infiltration, and may be a relevant target for drug therapy due to its limited expression in normal cell phenotypes.

SCC440
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC440
      DescriptionSK-MEL-147 Human Melanoma Cell Line 
      OverviewSK-MEL-147 is a human metastatic melanoma cell line. It retains a Q61R NRAS point mutation which is associated with a more aggressive phenotype, higher metastasis risk, and poorer prognosis.
      Alternate Names
      • human melanoma cell line
      • human melanoma cells
      • SK-MEL-147
      • metastatic melanoma cell line
      • Q61R NRAS melanoma cell line
      Background InformationMelanoma is considered the most dangerous form of skin cancer. It develops in melanocytes, the pigment producing cells of the skin. Melanoma accounts for a very small number of all skin cancers. Even though it accounts for only about 1% of total diagnosed skin cancer cases, melanoma is responsible for the largest number of skin cancer deaths.2 Melanoma incidence rates have increased over time, although mortality rates from melanoma have improved over the last decade due to advances in treatment.

      While there is enhanced understanding of BRAF-mutant melanoma and treatment strategies, therapeutic approaches to NRAS-mutant melanoma have been less successful. Development of immune therapies for NRAS-specific mutants remains highly relevant.

      SK-MEL-147 retains a Q61R NRAS point mutation which is associated with a more aggressive melanoma phenotype, higher metastasis risk, and therefore a poorer prognosis.1 While there has been an improvement in understanding BRAF-mutant melanoma and its therapeutic strategies, solutions to NRAS-mutant melanoma has remained limited.


      Source:
      SK-MEL-147 was derived from unspecified metastatic site in a patient with malignant melanoma. This cell line was developed by the LJ Old lab at Memorial Sloan Kettering Cancer Center.

      References
      1. Muñoz-Couselo E, Adelantado EZ, Ortiz C, García JS, Perez-Garcia J. 2017. NRAS-mutant melanoma: current challenges and future prospect. Onco Targets Ther. 10:3941-3947.
      2. Schadendorf D, Fisher DE, Garbe C, Gershenwald JE, Grob J-J, Halpern A, Herlyn M, Marchetti MA, McArthur G, Ribas A, et al. 2015. Melanoma. Nat Rev Dis Primers. 1:15003.
      3. Zhang S, Chen K, Liu H, Jing C, Zhang X, Qu C, Yu S. 2021. PMEL as a prognostic biomarker and negatively associated with immune infiltration in skin cutaneous melanoma (SKCM). J Immunother. 44(6):214-223.
      4. Kaufmann WK, Nevis KR, Qu P, Ibrahim JG, Zhou T, Zhou Y, Simpson DA, Helms-Deaton J, Cordeiro-Stone M, Moore DT, et al. 2008. Defective cell cycle checkpoint functions in melanoma are associated with altered patterns of gene expression. J Invest Dermatol. 128(1):175-187.
      5. Houghton AN, Brooks H, Cote RJ, Taormina MC, Oettgen HF, Old LJ. 1983. Detection of cell surface and intracellular antigens by human monoclonal antibodies. J Exp Med. 158(1): 53-65.
      References
      Product Information
      Components
      • ≥1x106 viable SK-MEL-147 Cells: (Catalog No. SCC440). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      Application The SK-MEL-147 cell line expresses PMEL and MelanA proteins, two common melanoma markers. PMEL has been implicated in poor immune cell infiltration, and may be a relevant target for drug therapy due to its limited expression in normal cell phenotypes.
      Key Applications
      • Cell Culture
      • Cell Function Assay
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      HostHuman
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥1X10⁶ viable cells.
      • SK-MEL-147 cells are verified to be of human origin and negative for mouse, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells tested negative for infectious diseases against a Contamination Clear panel by Charles River Animal Diagnostic Services.
      • Cells tested negative for mycoplasma.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsSK-MEL-147 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC440 04065270293756