Certificate of Analysis 1.52006 HX135905
- Document Type:
- Certificate of Analysis
- Lot Number:
- HX135905
- Product Catalog Number:
- 1.52006
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Certificate of Analysis 1.52006 HX937752
- Document Type:
- Certificate of Analysis
- Lot Number:
- HX937752
- Product Catalog Number:
- 1.52006
-
User Guide (IFU) - 1.52006
- Document Type:
- User Guide
- Product Catalog Number:
- 152006
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COA 152006
- Document Type:
- Certificate of Analysis
- Product Catalog Number:
- 152006
-
UHPLC 2 - Guidelines for high throughput separations
- Document Type:
- Brochure
- Product Catalog Number:
- Multiple
- Product Catalog Name:
- Multiple
-
Certificate of Analysis WX0001 52006
- Document Type:
- Certificate of Analysis
- Lot Number:
- 52006
- Product Catalog Number:
- WX0001
- Product Catalog Name:
- Water
-
Certificate of Analysis DX0831 52006
- Document Type:
- Certificate of Analysis
- Lot Number:
- 52006
- Product Catalog Number:
- DX0831
- Product Catalog Name:
- Dichloromethane
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IFT20 is required for opsin trafficking and photoreceptor outer segment development.
- The light-detecting outer segments of vertebrate photoreceptors are cilia. Like other cilia, all materials needed for assembly and maintenance are synthesized in the cell body and transported into the cilium. The highly elaborated nature of the outer segment and its high rate of turnover necessitate unusually high levels of transport into the cilium. In this work, we examine the role of the IFT20 subunit of the intraflagellar transport (IFT) particle in photoreceptor cells. IFT20 was deleted in developing cones by a cone-specific Cre and in mature rods and cones by a tamoxifen-activatable Cre. Loss of IFT20 during cone development leads to opsin accumulation in the inner segment even when the connecting cilium and outer segment are still intact. With time this causes cone cell degeneration. Similarly, deletion of IFT20 in mature rods causes rapid accumulation of rhodopsin in the cell body, where it is concentrated at the Golgi complex. We further show that IFT20, acting both as part of the IFT particle and independent of the particle, binds to rhodopsin and RG-opsin. Since IFT20 dynamically moves between the Golgi complex and the connecting cilium, the current work suggests that rhodopsin and opsins are cargo for IFT transport.
- Document Type:
- Reference
- Product Catalog Number:
- AB5405
- Product Catalog Name:
- Anti-Opsin Antibody, Red/Green
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HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair.
- Heterochromatin protein 1 (HP1), a major component of constitutive heterochromatin, is recruited to DNA damage sites. However, the mechanism involved in this recruitment and its functional importance during DNA repair remain major unresolved issues. Here, by characterizing HP1α dynamics at laser-induced damage sites in mammalian cells, we show that the de novo accumulation of HP1α occurs within both euchromatin and heterochromatin as a rapid and transient event after DNA damage. This recruitment is strictly dependent on p150CAF-1, the largest subunit of chromatin assembly factor 1 (CAF-1), and its ability to interact with HP1α. We find that HP1α depletion severely compromises the recruitment of the DNA damage response (DDR) proteins 53BP1 and RAD51. Moreover, HP1α depletion leads to defects in homologous recombination-mediated repair and reduces cell survival after DNA damage. Collectively, our data reveal that HP1α recruitment at early stages of the DDR involves p150CAF-1 and is critical for proper DNA damage signaling and repair.
- Document Type:
- Reference
- Product Catalog Number:
- Multiple
- Product Catalog Name:
- Multiple