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  • Olivocochlear suppression of outer hair cells in vivo: evidence for combined action of BK and SK2 channels throughout the cochlea. 23282326

    Cholinergic inhibition of cochlear hair cells via olivocochlear (OC)-efferent feedback is mediated by Ca(2+) entry through α9-/α10-nicotinic receptors, but the nature of the K(+) channels activated by this Ca(2+) entry has been debated (Yoshida N, Hequembourg SJ, Atencio CA, Rosowski JJ, Liberman MC. J Neurophysiol 85: 84-88, 2001). A recent in vitro study (Wersinger E, McLean WJ, Fuchs PA, Pyott SJ. PLoS One 5: e13836, 2010) suggests that small-conductance (SK2) channels mediate cholinergic effects in the apical turn, whereas large-conductance (BK) channels mediate basal turn effects. Here, we measure, as a function of cochlear frequency, the magnitude of BK and SK2 expression in outer hair cells and the strength of in vivo OC suppression in BK(+/+) mice vs. BK(-/-) lacking the obligatory α-subunit (Meredith AL, Thorneloe KS, Werner ME, Nelson MT, Aldrich RW. J Biol Chem 279: 36746-36752, 2004). Except at the extreme apical tip, we see immunostaining for both BK and SK2 in BK(+/+). Correspondingly, at all testable frequencies (8-45 kHz), we see evidence for both SK2 and BK contributions to OC effects evoked by electrically stimulating the OC bundle: OC-mediated suppression was reduced, but not eliminated, at all frequencies in the BK(-/-) ears. The suppression remaining in BK nulls was blocked by strychnine, suggesting involvement of α9-/α10-cholinergic receptors, coupled to activation of the remaining SK2 channels.
    Document Type:
    Reference
    Product Catalog Number:
    MAB397
    Product Catalog Name:
    Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4

  • Human papillomavirus E7 induces rereplication in response to DNA damage. 23152514

    Human papillomavirus (HPV) infection is necessary but not sufficient for cervical carcinogenesis. Genomic instability caused by HPV allows cells to acquire additional mutations required for malignant transformation. Genomic instability in the form of polyploidy has been demonstrated to play an important role in cervical carcinogenesis. We have recently found that HPV-16 E7 oncogene induces polyploidy in response to DNA damage; however, the mechanism is not known. Here we present evidence demonstrating that HPV-16 E7-expressing cells have an intact G(2) checkpoint. Upon DNA damage, HPV-16 E7-expressing cells arrest at the G(2) checkpoint and then undergo rereplication, a process of successive rounds of host DNA replication without entering mitosis. Interestingly, the DNA replication initiation factor Cdt1, whose uncontrolled expression induces rereplication in human cancer cells, is upregulated in E7-expressing cells. Moreover, downregulation of Cdt1 impairs the ability of E7 to induce rereplication. These results demonstrate an important role for Cdt1 in HPV E7-induced rereplication and shed light on mechanisms by which HPV induces genomic instability.
    Document Type:
    Reference
    Product Catalog Number:
    Multiple
    Product Catalog Name:
    Multiple

  • Anti-CDT1 - NG1806854

    Document Type:
    Certificate of Analysis
    Lot Number:
    NG1806854
    Product Catalog Number:
    07-1383
    Product Catalog Name:
    Anti-CDT1 Antibody

  • Anti-KLF9 - NRG1672067

    Document Type:
    Certificate of Analysis
    Lot Number:
    NRG1672067
    Product Catalog Number:
    06-1383
    Product Catalog Name:
    Anti-KLF9 Antibody

  • Anti-CDT1 Polyclonal Antibody

    Document Type:
    Certificate of Analysis
    Lot Number:
    2970628
    Product Catalog Number:
    07-1383
    Product Catalog Name:
    Anti-CDT1 Antibody

  • Anti-CDT1 - NG1618263

    Document Type:
    Certificate of Analysis
    Lot Number:
    NG1618263
    Product Catalog Number:
    07-1383
    Product Catalog Name:
    Anti-CDT1 Antibody

  • Anti-CDT1

    Document Type:
    Certificate of Analysis
    Lot Number:
    2476750
    Product Catalog Number:
    07-1383
    Product Catalog Name:
    Anti-CDT1 Antibody

  • Anti-CDT1 -2673041

    Document Type:
    Certificate of Analysis
    Lot Number:
    2673041
    Product Catalog Number:
    07-1383
    Product Catalog Name:
    Anti-CDT1 Antibody