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NE1039 Anti-IGFBP6 Mouse mAb (1H2-2D10)

NE1039
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the IGFBP6 protein in A431 cells.
      Catalogue NumberNE1039
      Brand Family Calbiochem®
      SynonymsAnti-Insulin-Like Growth Factor Binding Protein 6
      Application Data
      Detection of human IGFBP6 by immunoblotting. Sample: Whole cell lysate from A431 cells. Priamry antibody: Anti-IGFBP6 Mouse mAb (1H2-2D10) (Cat. No. NE1039) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlA431 cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution
      Biological Information
      ImmunogenFull-length, recombinant human IGFBP6 (aa 1-241), expressed as a GST fusion protein
      Clone1H2-2D10
      HostMouse
      IsotypeIgG2b
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NE1039 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Insulin-Like Growth Factor Binding Protein 6
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human IGFBP6 by immunoblotting. Sample: Whole cell lysate from A431 cells. Priamry antibody: Anti-IGFBP6 Mouse mAb (1H2-2D10) (Cat. No. NE1039) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the IGFBP6 protein.
      BackgroundIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
      HostMouse
      ImmunogenFull-length, recombinant human IGFBP6 (aa 1-241), expressed as a GST fusion protein
      Clone1H2-2D10
      IsotypeIgG2b
      Specieshuman
      Positive controlA431 cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review