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IM53 Anti-MMP-2 (Ab-6) Mouse mAb (CA-4001)

Anti-MMP-2 (Ab-6) Mouse mAb (CA-4001) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Gelatinase A, Anti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2

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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~72 kDa latent form of MMP-2 in HT1080 cells and breast carcinoma tissue. Does not cross-react with the latent or active forms of MMP-9.
      Catalogue NumberIM53
      Brand Family Calbiochem®
      SynonymsAnti-Gelatinase A, Anti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2
      Application Data
      Detection of human MMP-2 by immunoblotting. Sample: Human MMP-2. Primary antibody: Anti-MMP-2 (Ab-6) Mouse mAb (CA-4001) (Cat. No. IM53) (0.5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, 2, 99.
      Product Information
      DeclarationNot available for sale in Japan.
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Positive controlMMP-2 protein (Cat. Nos. 444213 or PF118), conditioned medium from endothelial cells, HT1080 cells, or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesFrozen Sections, Paraffin Sections Nikkari, S.T., et al. 1996. Am. J. Pathol. 149, 1427. Soini, Y., et al. 1994. J. Histochem. Cytochem. 42, 945. Autio-Harmainen, H., et al. 1993. Lab. Invest. 69, 312. Autio-Harmainen, H., et al. 1992. Lab. Invest. 67, 191. Margulies, I.M., et al. 1992. Cancer Epidemiol. Biomarkers Prev. 1, 467. Immunoblotting, Original Clone Margulies, I.M., et al. 1992. Cancer Epidemiol. Biomarkers Prev. 1, 467.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Not Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen sections (1:50-1:100, see application references)
      Immunoblotting (1:50-1:100, reducing only, see application references)
      Immunoprecipitation (not recommended)
      Paraffin Sections (1:50-1:100, see application references)
      Application CommentsThis antibody shows no cross-reactivity with the latent or active forms of MMP-9. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (APSPIIKFPGDVAPKTDK) corresponding to amino acids at the N-terminus of procollagenase IV
      ImmunogenHuman
      CloneCA-4001
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM53 0

      Documentation

      References

      Reference overview
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, 2, 99.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2008 JSW
      SynonymsAnti-Gelatinase A, Anti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2
      ApplicationFrozen sections (1:50-1:100, see application references)
      Immunoblotting (1:50-1:100, reducing only, see application references)
      Immunoprecipitation (not recommended)
      Paraffin Sections (1:50-1:100, see application references)
      Application Data
      Detection of human MMP-2 by immunoblotting. Sample: Human MMP-2. Primary antibody: Anti-MMP-2 (Ab-6) Mouse mAb (CA-4001) (Cat. No. IM53) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody (see application references). Recognizes a ~72 kDa of protein.
      BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin, and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus, and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-2 (Gelatinase A, 72 kDa gelatinase, type IV collagenase, TBE-1) is synthesized as a 631 amino acid proenzyme (72 kDa zymogen) which is proteolytically processed at the first 80 amino acids to the 66 kDa active form. MMP-2, along with its most closely related member of the MMP family, MMP-9, show substrate specificity toward type IV and V collagens, gelatin, and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential and suggest that MMP-2 may be a useful marker for the diagnosis or prognosis of cancer.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide (APSPIIKFPGDVAPKTDK) corresponding to amino acids at the N-terminus of procollagenase IV
      CloneCA-4001
      IsotypeIgG₁
      Specieshuman
      Positive controlMMP-2 protein (Cat. Nos. 444213 or PF118), conditioned medium from endothelial cells, HT1080 cells, or breast carcinoma tissue
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody shows no cross-reactivity with the latent or active forms of MMP-9. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, 2, 99.
      Application referencesFrozen Sections, Paraffin Sections Nikkari, S.T., et al. 1996. Am. J. Pathol. 149, 1427. Soini, Y., et al. 1994. J. Histochem. Cytochem. 42, 945. Autio-Harmainen, H., et al. 1993. Lab. Invest. 69, 312. Autio-Harmainen, H., et al. 1992. Lab. Invest. 67, 191. Margulies, I.M., et al. 1992. Cancer Epidemiol. Biomarkers Prev. 1, 467. Immunoblotting, Original Clone Margulies, I.M., et al. 1992. Cancer Epidemiol. Biomarkers Prev. 1, 467.