05-649 | Anti-Chk2 Antibody, clone 7

05-649
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-649
      ReplacesMABE491
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Chk2 Antibody, clone 7
      Alternate Names
      • CHK2 (checkpoint, S.pombe) homolog
      • CHK2 checkpoint homolog (S. pombe)
      • checkpoint-like protein CHK2
      • protein kinase CHK2
      • serine/threonine-protein kinase CHK2
      Background InformationThe p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate, HeLa cell lysate
        Included Positive Antigen Control: Catalog # 12-303, Jurkat Cell Lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationProtein G purified mouse IgG1 buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.
      Applications
      ApplicationAnti-Chk2 Antibody, clone 7 is a mouse monoclonal antibody for detection of Chk2 also known as CHK2 checkpoint homolog (S. pombe), serine/threonine-protein kinase CHK2 & has been validated in WB, IP, ICC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunoprecipitation: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Chk2 from HeLa cells.

      Immunocytochemistry: A previous lot of this antibody has been reported by an independent laboratory to detect Chk2 in HeLa cells.
      Biological Information
      ImmunogenMixture of two GST fusion proteins corresponding to residues 1-223 and 217-543 of human Chk2
      Cloneclone 7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityChk2
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and mouse. Others not tested. Predicted to cross-react with rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • CHEK2
      • HuCds1
      • CHK2
      • LFS2
      • RAD53
      • bA444G7
      • CDS1
      • OTTHUMP00000028871
      • PP1425
      • Cds1
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
      COFACTOR: Magnesium.
      SIZE: 543 amino acids; 60915 Da
      SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. & Isoform 4: Nucleus. & Isoform 7: Nucleus. & Isoform 9: Nucleus. & Isoform 12: Nucleus.
      TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
      DISEASE: SwissProt: O96017 # Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. & Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. & Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].
      SIMILARITY: SwissProt: O96017 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. & Contains 1 FHA domain. & Contains 1 protein kinase domain.
      Molecular Weight67 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from Jurkat cells, HeLa, 3T3/A31, or A431 cells.

      Western Blot Analysis: 0.1-1 μg/mL of this lot detected Chk2 in RIPA lysates from Jurkat cells. A previous lot detected Chk2 in lysates from HeLa, 3T3/A31, and A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Chk2, clone 7 - 2328347 2328347
      Anti-Chk2, clone 7 - 2446849 2446849
      Anti-Chk2, clone 7 - 1959677 1959677
      Anti-Chk2, clone 7 - 2020540 2020540
      Anti-Chk2, clone 7 - 2194158 2194158
      Anti-Chk2, clone 7 - 22186 22186
      Anti-Chk2, clone 7 - 28044 28044
      Anti-Chk2, clone 7 - DAM1530416 DAM1530416
      Anti-Chk2, clone 7 - DAM1641087 DAM1641087
      Anti-Chk2, clone 7 - DAM1692608 DAM1692608

      References

      Reference overviewApplicationPub Med ID
      Evaluation of novel imidazotetrazine analogues designed to overcome temozolomide resistance and glioblastoma regrowth.
      Ramirez, YP; Mladek, AC; Phillips, RM; Gynther, M; Rautio, J; Ross, AH; Wheelhouse, RT; Sakaria, JN
      Molecular cancer therapeutics  14  111-9  2015

      Show Abstract
      25351918 25351918
      Mir-23a induces telomere dysfunction and cellular senescence by inhibiting TRF2 expression.
      Luo, Z; Feng, X; Wang, H; Xu, W; Zhao, Y; Ma, W; Jiang, S; Liu, D; Huang, J; Songyang, Z
      Aging cell  14  391-9  2015

      Show Abstract
      25753893 25753893
      EXO1 is critical for embryogenesis and the DNA damage response in mice with a hypomorphic Nbs1 allele.
      Rein, K; Yanez, DA; Terré, B; Palenzuela, L; Aivio, S; Wei, K; Edelmann, W; Stark, JM; Stracker, TH
      Nucleic acids research  43  7371-87  2015

      Show Abstract
      26160886 26160886
      CHK1-driven histone H3.3 serine 31 phosphorylation is important for chromatin maintenance and cell survival in human ALT cancer cells.
      Chang, FT; Chan, FL; R McGhie, JD; Udugama, M; Mayne, L; Collas, P; Mann, JR; Wong, LH
      Nucleic acids research  43  2603-14  2015

      Show Abstract
      25690891 25690891
      Aberrant topoisomerase-1 DNA lesions are pathogenic in neurodegenerative genome instability syndromes.
      Katyal, S; Lee, Y; Nitiss, KC; Downing, SM; Li, Y; Shimada, M; Zhao, J; Russell, HR; Petrini, JH; Nitiss, JL; McKinnon, PJ
      Nature neuroscience  17  813-21  2014

      Show Abstract
      Western Blotting24793032 24793032
      Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and mitotic catastrophe.
      Sarbajna, S; Davies, D; West, SC
      Genes & development  28  1124-36  2014

      Show Abstract
      Western Blotting24831703 24831703
      Parvovirus-induced depletion of cyclin B1 prevents mitotic entry of infected cells.
      Adeyemi, RO; Pintel, DJ
      PLoS pathogens  10  e1003891  2014

      Show Abstract
      24415942 24415942
      HELQ promotes RAD51 paralogue-dependent repair to avert germ cell loss and tumorigenesis.
      Adelman, CA; Lolo, RL; Birkbak, NJ; Murina, O; Matsuzaki, K; Horejsi, Z; Parmar, K; Borel, V; Skehel, JM; Stamp, G; D'Andrea, A; Sartori, AA; Swanton, C; Boulton, SJ
      Nature  502  381-4  2013

      Show Abstract
      Western Blotting24005329 24005329
      The telomere deprotection response is functionally distinct from the genomic DNA damage response.
      Cesare, AJ; Hayashi, MT; Crabbe, L; Karlseder, J
      Molecular cell  51  141-55  2013

      Show Abstract
      23850488 23850488
      Conditional inactivation of the DNA damage response gene Hus1 in mouse testis reveals separable roles for components of the RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance.
      Lyndaker, AM; Lim, PX; Mleczko, JM; Diggins, CE; Holloway, JK; Holmes, RJ; Kan, R; Schlafer, DH; Freire, R; Cohen, PE; Weiss, RS
      PLoS genetics  9  e1003320  2013

      Show Abstract
      23468651 23468651

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      Included Positive Control

      Catalogue Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies