05-414 | Anti-CrkL Antibody, clone 5-6

05-414
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-414
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CrkL Antibody, clone 5-6
      Background InformationCRKL is a 39kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
      The antibody does not cross-react with CRKI or CRKII.
      References
      Product Information
      FormatPurified
      Control
      • Western Blot and Immunoprecipitation:
        K562 cell lysate
        Immunocytochemistry:
        Hela cells
      PresentationPurified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
      Applications
      ApplicationDetect CrkL using this Anti-CrkL Antibody, clone 5-6 validated for use in IP, WB, IH.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenFull length GST fusion protein corresponding to Human CRKL
      EpitopeFull-length
      Clone5-6
      HostMouse
      Specificitythe C-terminal SH3 of Human CRKL
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteReacts with human, mouse and rat. Other species have not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summaryv-CRK avian sarcoma virus CT10-homolog-like contains one SH2 domain and two SH3 domains. CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase and plays a role in fibroblast transformation by BCR-ABL. In addition, CRKL has oncogenic potential.
      Gene Symbol
      • CRKL
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P46109 # May mediate the transduction of intracellular signals.
      SIZE: 303 amino acids; 33777 Da
      SUBUNIT: Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB.
      SIMILARITY: SwissProt: P46109 ## Contains 1 SH2 domain. & Contains 2 SH3 domains.
      Molecular Weight39kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on K562 cell lysate.

      Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti- CRKL, Clone 5-6 2842900
      Anti- CRKL, Clone 5-6 - 1967296 1967296
      Anti- CRKL, Clone 5-6 - 1993877 1993877
      Anti- CRKL, Clone 5-6 - 2225228 2225228
      Anti- CRKL, Clone 5-6 - 2510221 2510221
      Anti- CRKL, Clone 5-6 - DAM1718037 DAM1718037
      Anti- CRKL, Clone 5-6 - DAM1828783 DAM1828783
      Anti- CRKL, Clone 5-6 - JBC1863406 JBC1863406
      Anti- CRKL, Clone 5-6 - NG1568501 NG1568501
      Anti- CRKL, Clone 5-6 -2586444 2586444

      References

      Reference overviewApplicationPub Med ID
      SIRT1 decreases Lox-1-mediated foam cell formation in atherogenesis.
      Stein S, Lohmann C, Schäfer N, Hofmann J, Rohrer L, Besler C, Rothgiesser KM, Becher B, Hottiger MO, Borén J, McBurney MW, Landmesser U, Lüscher TF, Matter CM
      Eur Heart J  2010

      Show Abstract
      20418343 20418343
      IL-4 regulates the expression of CD209 and subsequent uptake of Mycobacterium leprae by Schwann cells in human leprosy.
      Teles RM, Krutzik SR, Ochoa MT, Oliveira RB, Sarno EN, Modlin RL
      Infect Immun  2010

      Show Abstract
      20713631 20713631
      The early isoform of disabled-1 functions independently of Reelin-mediated tyrosine phosphorylation in chick retina.
      Gao, Z; Monckton, EA; Glubrecht, DD; Logan, C; Godbout, R
      Molecular and cellular biology  30  4339-53  2010

      Show Abstract
      20606009 20606009
      CrkL directs ASAP1 to peripheral focal adhesions
      Oda, A., et al
      J Biol Chem, 278:6456-60 (2003)  2003

      Immunocytochemistry12522101 12522101
      The proto-oncogene product p120CBL and the adaptor proteins CRKL and c-CRK link c-ABL, p190BCR/ABL and p210BCR/ABL to the phosphatidylinositol-3' kinase pathway
      Sattler, M, et al
      Oncogene, 12:839-46 (1996)  1996

      8632906 8632906
      Cellular interactions of CRKL, and SH2-SH3 adaptor protein
      ten Hoeve, J, et al
      Cancer Res, 54:2563-7 (1994)  1994

      8168080 8168080

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies