05-1583 | Anti-PRL-2 Antibody, clone 42

05-1583
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1583
      DescriptionAnti-PRL-2 Antibody, clone 42
      Alternate Names
      • Protein-tyrosine phosphatase 4a2
      • Protein-tyrosine phosphatase of regenerating liver 2
      • phosphatase of regenerating liver 2
      • protein tyrosine phosphatase IVA
      • protein tyrosine phosphatase IVA2
      • protein tyrosine phosphatase type IVA, member 2
      Background InformationPRL-2 is one of three members (PRL-1, PRL-2, and PRL-3) that is part of a unique protein-tyrosine phosphatase ((PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP is known to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferres a transformed phenotype and can result in a tumor growth in nude mice, suggesting a role in tumorigenesis. Alternatively spliced transcript variants that encode two distinct isoforms have been described
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-PRL-2 Antibody, clone 42 detects level of PRL-2 & has been published & validated for use in WB, IH & IP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis:
      A representative lot was used by an independent laboratory in IH.

      Immunoprecipitation Analysis:
      A representative lot was used by an independent laboratory in IP.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse PRL-2.
      EpitopeUnknown
      Clone42
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes PRL-2.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ptp4a2
      • HH13
      • HH7-2
      • HU-PP-1
      • OV-1
      • PRL-2
      • PRL2
      • PTP(CAAXII)
      • PTP4A
      • PTPCAAX2
      • ptp-IV1a
      • ptp-IV1b
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyltransferase type II activity by blocking the association between RABGGTA and RABGGTB By similarity.
      CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
      ENZYME REGULATION: Inhibited by sodium orthovanadate and pentamidine By similarity.
      SUBUNIT STRUCTURE: In contrast to PTP4A1 and PTP4A3, does not interact with tubulin. Interacts with RABGGTB By similarity.
      SUBCELLULAR LOCATION: Cell membrane. Early endosome. Cytoplasm
      TISSUE SPECIFICTY: Expressed in skeletal muscle, and at lower levels in liver, lung, heart, kidney, brain, testis and spleen. Ref.1
      PTM: Farnesylated. Farnesylation is required for membrane targeting and for interaction with RABGGTB By similarity.
      SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family.
      Contains 1 tyrosine-protein phosphatase domain.
      Molecular Weight~ 19 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.
      Western Blot Analysis: 0.5 µg/ml of this antibody detected PRL-2 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PRL-2, clone 42 2476754
      Anti-PRL-2, clone 42 - 2202494 2202494
      Anti-PRL-2, clone 42 - NRG1690535 NRG1690535
      Anti-PRL-2, clone 42 -2660180 2660180
      Anti-PRL-2, clone 42 -2786962 2786962
      Anti-PRL-2, clone 42 -2894078 2894078
      Anti-PRL-2, clone 42 Monoclonal Antibody 3003442
      Anti-PRL-2, clone 42, Monoclonal Antibody 2839585
      Anti-PRL-2, clone 42_2849468 2849468

      References

      Reference overviewPub Med ID
      Cellular proteins associated with the interior and exterior of vesicular stomatitis virus virions.
      Moerdyk-Schauwecker, M; Hwang, SI; Grdzelishvili, VZ
      PloS one  9  e104688  2014

      Show Abstract
      25105980 25105980