05-1478 | Anti-RUNX2 Antibody, clone AS110

05-1478
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1478
      DescriptionAnti-RUNX2 Antibody, clone AS110
      Alternate Names
      • Acute myeloid leukemia 3 protein
      • CBF-alpha 1
      • Core-binding factor subunit alpha-1
      • Oncogene AML-3
      • Osteoblast-specific transcription factor 2
      • PEA2-alpha A
      • PEBP2-alpha A
      • Polyomavirus enhancer-binding protein 2 alpha A subunit
      • SL3-3 enhancer factor 1 alpha A subunit
      • SL3/AKV core-binding factor alpha A subunit, core-binding factor, runt domain, alpha subunit 1
      • polyomavirus enhancer binding protein 2 alpha A subunit
      • runt-related transcription factor 2
      Background InformationRUNX2 (Runt-related transcription Factor) is a transcription factor that binds to its canonical consensus sequence 5’- PYGPYGGT-3’ in a number of promoters and enhancers. They include LCK, T-cell receptor enhancers, and various bone related genes such as osteocalcin, osteopontin, and bone sialoprotein. As a result of this, RUNX2 is involved in osteoblastic differentiation and skeletal morphogenesis. RUNX2 contains a proline/serine/ threonine-rich region at its C-terminus that has shown to be critical for its transcriptional activity.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4) with 150mM NaCl and 0.05% NaN3.
      Applications
      ApplicationAnti-RUNX2 Antibody, clone AS110 detects level of RUNX2 & has been published & validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST-coupled recombinant protein corresponding to amino acids 311-415 of human RUNX2.
      EpitopeInternal
      CloneAS110
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityDetects RUNX2
      IsotypeIgG2b
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Other species have not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq]
      Gene Symbol
      • AML3
      • CBF-alpha-1
      • CBFA1
      • CCD
      • CCD1
      • MGC120022
      • MGC120023
      • OSF-2
      • OSF2
      • OTTHUMP00000016533
      • PEA2aA
      • PEBP2A
      • PEBP2A1
      • PEBP2A2
      • PEBP2aA
      • PEBP2aA1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. Inhibits MYST4-dependent transcriptional activation.

      SUBUNIT: Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 By similarity. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with G22P1 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4.

      SUBCELLULAR LOCATION: Nucleus.

      SPECIFICITY: Specifically expressed in osteoblasts.

      DOMAIN: A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites.

      PTM: Phosphorylated; probably by MAP kinases (MAPK) By similarity. Isoform 3 is phosphorylated on Ser-340.

      DISEASE: Defects in RUNX2 are the cause of cleidocranial dysplasia (CCD) [MIM:119600]. CCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. Ref.1 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

      SIMILARITY:Contains 1 Runt domain.

      Molecular Weight54-57 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot.

      Western Blot Analysis:
      Anti-RUNX2, clone AS110 detected RUNX at 1:1,000 to 1:2,000 dilution in HL-60 cell lysate resolved via SDS-PAGE and transferred to PVDF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RUNX2, clone AS110 - 2136648 2136648
      Anti-RUNX2, clone AS110 - 2376704 2376704
      Anti-RUNX2, clone AS110 - 2430528 2430528
      Anti-RUNX2, clone AS110 - NG1818071 NG1818071
      Anti-RUNX2, clone AS110 - NG1845590 NG1845590
      Anti-RUNX2, clone AS110 - 2007358 2007358
      Anti-RUNX2, clone AS110 - 2299609 2299609
      Anti-RUNX2, clone AS110 - NG1572389 NG1572389
      Anti-RUNX2, clone AS110 - NG1637563 NG1637563
      Anti-RUNX2, clone AS110 - NG1748987 NG1748987

      References

      Reference overviewApplicationPub Med ID
      Vascular Klotho deficiency potentiates the development of human artery calcification and mediates resistance to fibroblast growth factor 23.
      Lim, K; Lu, TS; Molostvov, G; Lee, C; Lam, FT; Zehnder, D; Hsiao, LL
      Circulation  125  2243-55  2012

      Show Abstract
      Western Blotting22492635 22492635