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Bacterial Cell Lysis

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Featured Application Note
Simplifying the Bacterial Recombinant Protein Workflow while Maximizing Recovery
A Complete Solution from Expression to Detection
BugBuster® Protein Extraction Reagent is formulated to gently disrupt the cell wall of E.coli and liberate soluble proteins. This flagship reagent from Merck utilizes a detergent mix that is capable of cell wall perforation without denaturing soluble protein. Simply add the BugBuster® Reagent to your cell pellet and incubate at room temperature for 10 minutes! No special preparation of your samples is required. No instruments are needed. Unlike other mechanical methods such as Sonication, protein denaturation is not a problem. BugBuster® reagent produces high yields that are reproducible, convenient and fast!  

Product Advantages:
  • Standard reagent is supplied as a Tris-buffered "1X" ready-to-use liquid
  • Stable at room temperature
  • Performance can be enhanced using Benzonase® nuclease and rLysozyme™ solution
BugBuster® reagent is superior to "Home Brew" lysis buffer and BugBuster® reagent with both Benzonase® nuclease and rLysozyme solution yielded lysates with the most 6XHis-CRP. (A) E.coli lysates (5μL of 1 mL total lysate) from various lysis protocols were fractionated and analyzed by SDS-PAGE. A band correspondinf to 6XHIS-CRP is prominently visualized in the BB +/+ lane.

BugBuster® Master Mix combines BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme Solution in one convenient reagent. BugBuster® plus Benzonase® includes BugBuster® Protein Extraction Reagent and Benzonase® Nuclease as two separate components (500mL and 10 KU respectively) in a single kit. BugBuster® Plus Lysonase includes BugBuster® Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimized, ready-to-use blend of rLysozyme and Benzonase® Nuclease in a single kit. BugBuster® 10X is an unbuffered concentrated formulation of the BugBuster® Protein Extraction Reagent offering you the flexibility to customize your dilution and buffer composition.
BugBuster® Master Mix versus Homebrew
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