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Merck

PMEF-CFX

EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Irradiated, Passage 3

Synonyme(s) :

CF-1 MEFs, CF-1 PMEFs, Irradiated MEFs, MEF Feeder Cells, MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells, Irradiated MEFS, Mouse Feeder Cells

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A propos de cet article

UNSPSC Code:
41106509
NACRES:
NA.81
eCl@ss:
32011203
Biological source:
mouse

Principaux documents

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biological source

mouse

manufacturer/tradename

Specialty Media, EmbryoMax®

technique(s)

cell culture | stem cell: suitable

input

sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)

shipped in

liquid nitrogen

Quality Level

100

storage temp.

-140 to -196°C

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Catégories apparentées

General description

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
These PMEF cells were isolated from CF-1 mouse embryos at day 13 and mitotically inactivated by treatment with irradiation. The cells can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:

• 6 x 60mm dishes
• 2 x T75 flasks
• 2 x 6 well plates

Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells.

Application

Research Category
Stem Cell Research

Biochem/physiol Actions

Primary Mouse Embryo Fibroblasts

Packaging

5-6x106 ea

Preparation Note

Vials should be stored in the vapor phase of liquid nitrogen.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

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Megan Bowers et al.
Cell stem cell, 27(1), 98-109 (2020-05-11)
Altered neural stem/progenitor cell (NSPC) activity and neurodevelopmental defects are linked to intellectual disability. However, it remains unclear whether altered metabolism, a key regulator of NSPC activity, disrupts human neurogenesis and potentially contributes to cognitive defects. We investigated links between

Contenu apparenté

The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

"As transgenic mouse core laboratories know well, mouse models are a powerful means of determining the function of mammalian genes in the context of whole organisms—especially since over 95% of the mouse genome is similar to the human genome. However, you may not know that: 1. Laboratory mouse strains are descended from ancestors in modern-day Pakistan. 2. The first inbred strain of lab mouse was created in 1909. It was named DBA for its coat color: dilute brown non-agouti. 3. There is a mutant mouse actually named“Bad Hair Day” (Bhrd). Someone get this little guy a stylist! 4. Male mice, when treated with anticancer drugs, have been shown to pass on DNA instability to their offspring. 5. The founder of Jackson Laboratories, C. C. Little, once had lunch with Walt Disney to request that an animated film be created linking Mickey Mouse and the laboratory mouse. The request was denied."

Numéro d'article de commerce international

RéférenceGTIN
PMEF-CFX04053252923661

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