A4929
N,N′-Bis(acryloyl)cystamine
BioReagent, suitable for electrophoresis
Synonyme(s) :
2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide
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A propos de cet article
Formule empirique (notation de Hill) :
C10H16N2O2S2
Numéro CAS:
Poids moléculaire :
260.38
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12352116
EC Number:
262-546-8
MDL number:
Form:
powder
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InChI key
DJVKJGIZQFBFGS-UHFFFAOYSA-N
InChI
1S/C10H16N2O2S2/c1-3-9(13)11-5-7-15-16-8-6-12-10(14)4-2/h3-4H,1-2,5-8H2,(H,11,13)(H,12,14)
SMILES string
C=CC(=O)NCCSSCCNC(=O)C=C
product line
BioReagent
form
powder
suitability
suitable for electrophoresis
storage temp.
−20°C
Application
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.
BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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