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Merck

FLAA

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit

for ATP quantitation

Synonyme(s) :

ATP Bioluminescence Assay, ATP Determination Kit, Luminescent ATP Detection kit

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A propos de cet article

EC Number:
213-579-1
NACRES:
NA.26
eCl@ss:
32160414
UNSPSC Code:
41116133

Principaux documents

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Nom du produit

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit, for ATP quantitation

storage temp.

−20°C

Quality Level

200

Catégories apparentées

Application

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit has also been used in the quantification of ATP in 3D matrixes of human neurons, hepatic cells with ischemia, various bacterial cultures and lysosomes.

General description

The Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of ATP in samples. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin. When ATP is the limiting reagent, the light emitted is proportional to the ATP present in the sample.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • FLAABAdenosine 5′-triphosphate (ATP) assay mix dilution buffer, lyophilized powder mL/vialFDS
  • FLAAMAdenosine 5′-triphosphate (ATP) assay mix, lyophilized powder mL/vialFDS
  • FLAASAdenosine 5′-triphosphate (ATP) disodium salt hydrate, vial of ~1 mg ATP mL/vialFDS

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

wgk

WGK 3

Classe de stockage

11 - Combustible Solids

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Certificats d'analyse (COA)

Lot/Batch Number
0000502396
0000502395
0000502396
0000502395
0000502399

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

  1. How many reactions can one do with Product FLAA, ATP Bioluminescent Assay Kit (one vial of FLAAM)?

    When one reconstitutes the vial to 5 ml, as directed, one can perform 50 assays using 100 ul per test. If one dilutes 1:5 one can get 250 tests per kit.

  2. What is the sensitivity of Product FLAA, ATP Bioluminescent Assay Kit the assay mix?

    With FLAAM diluted 1:5, one can easily measure 1 pmol ATP in 100 μl (10 nM) with 50 μl of the reagent.

  3. What  is the stability of the Product FLAA, ATP Bioluminescent Assay Kit ATP stock solution?

    The ATP standard stock solution (FLAAS) is stable for at least 24 hours at 0-5°C, or for over two weeks if stored frozen at -20°C.

  4. Can Product FLAA, ATP Bioluminescent Assay Kit assay mix be used on cells and tissues?

    This reagent measures only soluble ATP. Cells must be washed and then lysed to obtain a soluble ATP extract. Tissues must be homogenized and clarified to provide an extract. The supernatant solution can then be used for assay.

  5. How can one check for inhibitors in the samples when using Product FLAA, ATP Bioluminescent Assay Kit?

    One must add an amount of ATP from a standard solution equivalent to the ATP already found in the sample. If the signal is doubled, the absence of an inhibitor is indicated.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Witkowska A M, et al.
Journal of Food Research, 2(4), 37-37 (2013)
Xi Zoë Zhong et al.
The Journal of physiology, 594(15), 4253-4266 (2016-08-02)
SLC17A9 proteins function as a lysosomal ATP transporter responsible for lysosomal ATP accumulation. P2X4 receptors act as lysosomal ion channels activated by luminal ATP. SLC17A9-mediated ATP transport across the lysosomal membrane is suppressed by Bafilomycin A1, the V-ATPase inhibitor. SLC17A9
Marco A Tavares et al.
Environmental toxicology and pharmacology, 40(1), 206-214 (2015-07-06)
Fipronil is an insecticide extensively used to control pests in crops and animals. There are relates of poisoning due to exposure of fipronil in mammals and the liver has been suggested as potential target. In this study, we evaluated the
John F C Steele et al.
PloS one, 14(8), e0221226-e0221226 (2019-08-29)
Plant NLRs are modular immune receptors that trigger rapid cell death in response to attempted infection by pathogens. A highly conserved nucleotide-binding domain shared with APAF-1, various R-proteins and CED-4 (NB-ARC domain) is proposed to act as a molecular switch
Hynek Mergental et al.
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 24(10), 1453-1469 (2018-10-26)
Increased use of high-risk allografts is critical to meet the demand for liver transplantation. We aimed to identify criteria predicting viability of organs, currently declined for clinical transplantation, using functional assessment during normothermic machine perfusion (NMP). Twelve discarded human livers
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