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Merck

L3289

LYSIS SOLUTION FOR BLOOD

sufficient for 100 reactions, Molecular Biology

Synonyme(s) :

Blood direct PCR, Blood lysis buffer, Extract-N-Amp blood direct PCR lysis solution

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A propos de cet article

NACRES:
NA.25
UNSPSC Code:
12352200
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grade

Molecular Biology

Quality Level

form

liquid

usage

sufficient for 100 reactions

storage temp.

−20°C

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General description

Lysis solution for blood in combination with neutralization solution for blood (N9784) aids in rapid extraction and neutralization of DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

This lysis solution is a component of the Extract-N-Amp and the REDExtract-N-Amp Blood PCR Kits

Application

Lysis solution for blood has been used for lysis of blood cells for DNA extraction prior to polymerase chain reaction (PCR) amplification. It has also been used for the isolation of genomic DNA for CRISPR-Cas9 gene editing.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Classe de stockage

8A - Combustible corrosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and Cellular Biology, MCB-00790 (2015)
Shuhan Chen et al.
Stem cell research, 45, 101804-101804 (2020-04-28)
Mutations in the Leucine rich repeat kinase 2 (LRRK2) gene are found in both familial and sporadic Parkinson's disease (PD), and are also associated with immune-related disorders including Crohn's disease (CD) and leprosy. We have generated two homozygous LRRK2 knockout
Sabrina M Rodriguez et al.
The Journal of general virology, 90(Pt 11), 2788-2797 (2009-07-10)
Previous studies have classified the env sequences of bovine leukemia virus (BLV) provirus from different locations worldwide into between two and four genetic groupings. These different studies gave unique names to the identified groups and no study has yet integrated
Leandro S Thiago et al.
Haematologica, 99(1), 155-162 (2013-07-23)
The B-cell compartment in which multiple myeloma stem cells reside remains unclear. We investigated the potential presence of mature, surface-membrane immunoglobulin-positive B lymphocytes clonally related to the tumor bone marrow plasma cells among different subsets of peripheral blood B cells
G Henderson et al.
Archives of disease in childhood. Fetal and neonatal edition, 94(2), F124-F128 (2007-09-05)
The inflammatory cytokine cascade is implicated in the pathogenesis of necrotising enterocolitis (NEC). Genetic association studies of cytokine polymorphisms may help to detect molecular mechanisms that are causally related to the disease process. To examine associations between the common genetic

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