M1302
M-MLV Reverse Transcriptase
Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis
Synonyme(s) :
Moloney Murine Leukemia Virus Reverse Transcriptase
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A propos de cet article
Biological source:
Porcine intestinal mucosa
Recombinant:
expressed in E. coli
Concentration:
200 units/μL
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Laissez-nous vous aiderbiological source
Porcine intestinal mucosa
recombinant
expressed in E. coli
form
liquid
usage
sufficient for 200 reactions
feature
dNTPs included: no, hotstart: no
concentration
200 units/μL
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
Quality Level
Catégories apparentées
General description
Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.
Application
M-MLV Reverse Transcriptase has been used:
- for the preparation of cDNA libraries or for first strand cDNA synthesis
- for use in a 2-step RT-PCR assay
- in quantitative realtime-polymerase chain reaction (RT-qPCR)
- in reverse transcription
Features and Benefits
- Thermostable reverse transcriptase active at 37 °C.
- Can be used to generate first strand cDNA of up to 7 kb.
Packaging
Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.
Preparation Note
The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.
Other Notes
One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Murine leukemia virus reverse transcriptase: structural comparison with HIV-1 reverse transcriptase.
Marie L Coté et al.
Virus research, 134(1-2), 186-202 (2008-02-26)
Recent X-ray crystal structure determinations of Moloney murine leukemia virus reverse transcriptase (MoMLV RT) have allowed for more accurate structure/function comparisons to HIV-1 RT than were formerly possible. Previous biochemical studies of MoMLV RT in conjunction with knowledge of sequence
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