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Merck

NA0200

GenElute™ HP Plasmid Midiprep Kit

greener alternative

sufficient for 25 purifications

Synonyme(s) :

GenElute™ HP Plasmid Midiprep Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute

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A propos de cet article

NACRES:
NA.52
UNSPSC Code:
41105501

Principaux documents

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usage

sufficient for 25 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

Quality Level

200

test parameters

sample volume: 1-5 mL bacterial culture

greener alternative category

, Aligned

storage temp.

15-25°C

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Catégories apparentées

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
Click here to view the kit protocol

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Application

GenElute™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.

Features and Benefits

  • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
  • Up to 350 μg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • No phenol/chloroform extraction or alcohol precipitation required
  • Contains fewer plastic components, reducing the amount of waste

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • R1149Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )FDS
  • W0263Wash Solution 1FDS
  • W4639Wash Solution 2FDS
  • R6148RNase A solutionFDS
  • C2112Column Preparation SolutionFDS
  • L1912Lysis SolutionFDS

signalword

Danger

target_organs

Central nervous system

Classe de stockage

3 - Flammable liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

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Certificats d'analyse (COA)

Lot/Batch Number
0000476568
0000476568
0000385570
0000333635
0000385570

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Consulter la Bibliothèque de documents

Vanessa E Gray et al.
G3 (Bethesda, Md.), 9(11), 3683-3689 (2019-09-29)
Despite the importance of Aβ aggregation in Alzheimer's disease etiology, our understanding of the sequence determinants of aggregation is sparse and largely derived from in vitro studies. For example, in vitro proline and alanine scanning mutagenesis of Aβ40 proposed core
Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
Steinhart Z
Nature Medicine, 23(1), 60-68 (2017)
The distal upstream promoter in Ly49 genes, Pro1, is active in mature NK cells and T cells, does not require TATA boxes, and displays enhancer activity.
Gays F
Journal of Immunology, 194(12), 6068-6081 (2015)
Stephanie P Cartwright et al.
BMC research notes, 10(1), 340-340 (2017-07-29)
Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV
Theresa B Loveless et al.
Nature chemical biology, 17(6), 739-747 (2021-03-24)
Studying cellular and developmental processes in complex multicellular organisms can require the non-destructive observation of thousands to billions of cells deep within an animal. DNA recorders address the staggering difficulty of this task by converting transient cellular experiences into mutations
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