T6763
Trypsin Acetylated from bovine pancreas
Type V-S, ≥8,500 BAEE units/mg protein (biuret)
Synonyme(s) :
Acetyltrypsin
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Trypsin Acetylated from bovine pancreas, Type V-S, ≥8,500 BAEE units/mg protein (biuret)
biological source
bovine pancreas
type
Type V-S
form
powder
specific activity
≥8,500 BAEE units/mg protein (biuret)
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
cow ... TRYP8(282603)
Catégories apparentées
Biochem/physiol Actions
Lys-acetylated trypsin is less susceptible to autolysis than the native form.
General description
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Application
Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.
Other Notes
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.
Preparation Note
Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Amy R Quinn et al.
The Journal of endocrinology, 214(3), 289-299 (2012-06-23)
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Proceedings of the National Academy of Sciences of the United States of America, 109(27), 10832-10836 (2012-06-20)
The study of membrane proteins remains a challenging task, and approaches to unravel their dynamics are scarce. Here, we applied hydrogen/deuterium exchange (HDX) coupled to mass spectrometry to probe the motions of a bacterial multidrug ATP-binding cassette (ABC) transporter, BmrA
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The development of a live-attenuated vaccine (LAV) for the prevention of human metapneumovirus (HMPV) infection is often hampered by the lack of highly efficient and scalable cell-based production systems that support eventual global vaccine production. Avian cell lines cultivated in
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