Kits et réactifs pour triglycérides et glycérol à l′état libre

Les kits pour triglycérides et glycérol à l′état libre sont utilisés pour la détermination quantitative du glycérol, des triglycérides totaux et des triglycérides à l′état libre.

A Serum Triglyceride Determination Kit was used to determine the level of triglycerides in whole-body homogenates of Drosophila melanogaster.

Suitable for the quantitative determination of glycerol, total triglycerides or free triglycerides in serum or plasma

The Serum Triglyceride Determination Kit can be used for the measurement of glycerol, true triglycerides, or total triglycerides in serum or plasma. The procedure involves enzymatic hydrolysis by lipase of the triglycerides to glycerol and free fatty acids. The glycerol produced is then measured by coupled enzyme reactions. Many of the triglyceride reagents which are commercially available, do not differentiate between endogenous glycerol and glycerol derived by hydrolytic action of lipase on glycerides.
Triglycerides are first hydrolyzed by lipoprotein lipase to glycerol and free fatty acids. Glycerol is then phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxy-acetone phosphate (DAP) and hydrogen peroxide (H₂O₂). Peroxidase (POD) catalyzes the coupling of H₂O₂ with 4-aminoantipyrine (4-AAP) and sodium
N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to triglyceride concentration of the sample.

En plus des kits, les réactifs individuels et le standard de glycérol sont disponibles pour réaliser un plus petit nombre de réactions.

The Serum Triglyceride Determination Kit can be used for the measurement of glycerol, true triglycerides, or total triglycerides in serum or plasma. The procedure involves enzymatic hydrolysis by lipase of the triglycerides to glycerol and free fatty acids. The glycerol produced is then measured by coupled enzyme reactions. Many of the triglyceride reagents which are commercially available, do not differentiate between endogenous glycerol and glycerol derived by hydrolytic action of lipase on glycerides. Triglycerides are first hydrolyzed by lipoprotein lipase to glycerol and free fatty acids. Glycerol is then phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxy-acetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase (POD) catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to triglyceride concentration of the sample.

The Serum Triglyceride Determination Kit is for the quantitative enzymatic measurement of glycerol, true triglycerides, and total triglycerides in serum or plasma at 540 nm. Triglycerides, esters of fatty acids and glycerol,1 do not circulate freely in plasma, but are bound to proteins and transported as macromolecular complexes called lipoproteins.2 Methods for triglyceride determination generally involve enzymatic3 or alkaline4 hydrolysis of triglycerides to glycerol and free fatty acids, followed by either chemical or enzymatic measurement of the glycerol released.

The kit also includes sufficient reagent for an additional 250 free glyceride tests for true triglyceride determination.