IROA TruQuant Yeast Extract Workflow Kit

TruQuant IQQ is a high-quality quantitation system for making simultaneous accurate biological measurements on several hundred biochemicals in small quantities of biological samples. This is achieved by (1) spiking a complex Internal Standard (WORKFLOW-A) into a biological sample to a) quantify all the biochemicals in the sample relative to their counterparts in the Internal Standard, b) suppression-correct each compound and c) normalize sample to sample variances; and (2) injecting the same well characterized Long-Term Reference Standard (WORKFLOW-B) to create a daily retention time (RT) library of all compounds to be found in the Internal Standard for reproducible ID, and to measure day-to-day (QA/QC) to assure reproducible instrument performance. The system is completely automated using IROA ClusterFinder^™ software.

IROA TruQuant IQQ Workflow Kit contains the materials and tools for the analysis of 90 experimental samples. The kit is intended to be used for mass spectrometry metabolomics applications.

Identification: Confidence in identifying common and new metabolites
Quantification: Comprehensive correction of analytical and biological variability for all analytes
Quality Control: Convenient and automated tools for assuring reproducibility within/across studies

• Simultaneous metabolite identification, quantitation and platform QC
• Accurate compound formula ID for MS alone; complete ID with addition of SWATH, or IM
• IROA patterns discriminate peaks of biological origin from artefactual peaks allowing the removal of false data
• IROA-based fragments have the IROA ratio pattern of their parent peaks and can be identified as such using the “peak correlation” ClusterFinder^™ module
• Retention Index within the sample allows “unknowns” to be tracked with precision
• Suppression-corrected measurements for significantly better quantitation
• Normalizes (suppression-corrected) experimental samples to overcome day-to-day, instrument-to-instrument variances
• ClusterFinder^™ software solution builds libraries, IDs/quantitates compounds and normalizes data
• Ensures high level QC for accurate and reproducible results

ClusterFinder^™ software package is distributed with and is tailored to work with the IROA TruQuant IQQ kit. A user manual and video instructions are provided.
To download the software package, click here - ClusterFinder software.
Link to the ClusterFinder^™ Videos below:

• IROA Peaks Basics
• CF Process Overview
• CF Initiation
• CF New Project & Add Data
• CF Unbiased Search for IROA peaks
• CF Libraries & Methods
• CF BinTree - bins are displayed as a tree
• CF Curation 1: Untargeted - quality of the peaks
• CF Curation 2: Cascade

To access the MS Calculator Cascade Spreadsheet, follow the link below:
MS Calculator

For additional information on our range of Biochemicals, please complete this form.

ClusterFinder is a trademark of IROA Technologies LLC