XNAR
Extract-N-Amp™ Plant PCR Kit
sufficient for 1000 extractions, sufficient for 1000 amplifications
Synonyme(s) :
Plant direct PCR kit
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NACRES:
NA.55
UNSPSC Code:
41106303
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Laissez-nous vous aiderNom du produit
Extract-N-Amp™ Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
usage
sufficient for 1000 amplifications
sufficient for 1000 extractions
feature
hotstart
technique(s)
PCR: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Catégories apparentées
Application
Extract-N-Amp™ Plant PCR Kit has been used for DNA extraction from fresh mushrooms.
Extract-N-Amp™ Plant PCR Kit is suitable for use in genotyping.
It has been used forDNA extraction from fresh mushrooms.
It has been used forDNA extraction from fresh mushrooms.
Features and Benefits
- Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
- A PCR ReadyMix™, specially formulated for amplification directly from extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- Extract stable at 4 °C for at least 6 months
- Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
- No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
- A specially formulated PCR ReadyMix is provided for use with the extracted DNA
- The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
- Suitable for high-throughput plant genetic analysis.
- Lengthy enzymatic digestions are not required, saving time and effort.
- The extracted DNA remains stable at 4 °C for atleast 6 months.
General description
The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. The kit also includes a PCR ReadyMix™, especially formulated for amplification directly from extract.
Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit.
Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit.
The Extract-N-Amp™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.
Other Notes
Extract-N-Amp™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
flash_point_c
Not applicable
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
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M Riahi et al.
Genetics and molecular research : GMR, 9(3), 1334-1342 (2010-07-21)
Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with
Alison Heffer et al.
EvoDevo, 2(1), 7-7 (2011-03-03)
Tremendous progress has been made in the field of evo-devo through comparisons of related genes from diverse taxa. While the vast number of species in nature precludes a complete analysis of the molecular evolution of even one single gene family
Rapid and reliable high-throughput methods of DNA extraction for use in barcoding and molecular systematics of mushrooms
Dentinger BTM, et al.
Molecular Ecology Resources, 10(4), 628-633 (2010)
Antoine Lf Gady et al.
Plant methods, 5, 13-13 (2009-10-09)
The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as
Elena A Filipenko et al.
Physiology and molecular biology of plants : an international journal of functional plant biology, 17(1), 79-86 (2011-03-01)
Spectinomycin resistant mutant carrot (Daucus carota L.) callus lines detected in the experiments on biolistic transformation of plastome were analyzed. It has been found that this antibiotic resistance is determined by point nucleotide substitutions at two distinct sites of the
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