D3035

Deoxyribonucleic acid from human placenta

Name: Deoxyribonucleic acid from human placenta
Brand: SIGMA

buffered aqueous solution, sexed, female

Synonym(s):

DNA

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About This Item

CAS Number:

9007-49-2

UNSPSC Code:

41106310

NACRES:

NA.52

MDL number:

MFCD00130921

Product Name

Deoxyribonucleic acid from human placenta, buffered aqueous solution, sexed, female

SMILES string

[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

biological source

human placenta

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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Related Categories

Biochemicals

Chemistry & Biochemicals

Nucleosides and Nucleotides

Application

Deoxyribonucleic acid from human placenta has been used:

to compare the efficiency of DNA quantification methods
as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
as an internal control to estimate the degree of fragmentation of circulating DNA
for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
to compare the 70-bp P5 exon sequence between DNA of different human and primate species
to calculate copy number of genes, relative to normal human tissue
in PCR reactions

For use in Southern hybridizations.

General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Other Notes

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

wgk

WGK 3

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Origin and quantification of circulating DNA in mice with human colorectal cancer xenografts.

Alain R Thierry et al.

Nucleic acids research, 38(18), 6159-6175 (2010-05-25)

Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an

Measurement of relative copy number of CDKN2A/ARF and CDKN2B in bladder cancer by real-time quantitative PCR and multiplex ligation-dependent probe amplification.

Joanne S Aveyard et al.

The Journal of molecular diagnostics : JMD, 6(4), 356-365 (2004-10-28)

Many tumors have large homozygous deletions of the CDKN2A locus (encoding p14(ARF) and p16) and of CDKN2B (p15). Our aim was to determine which gene is the major target in bladder cancer. We used quantitative real-time PCR (RTQ-PCR) to determine

Quantitative Proteomic Verification of Membrane Proteins as Potential Therapeutic Targets Located in the 11q13 Amplicon in Cancers.

Heather Hoover et al.

Journal of proteome research, 14(9), 3670-3679 (2015-07-08)

Tumor types can be defined cytologically by their regions of chromosomal amplification, which often results in the high expression of both mRNA and proteins of certain genes contained within the amplicon. An important strategy for defining therapeutically relevant targets in

A tissue-specific promoter derived from a SINE retrotransposon drives biallelic expression of PLAGL1 in human lymphocytes.

Claire E L Smith et al.

PloS one, 12(9), e0185678-e0185678 (2017-09-29)

The imprinted gene PLAGL1 is an important regulator of apoptosis and cell cycle arrest. Loss of its expression has been implicated in tumorigenesis in a range of different cancers, and overexpression during fetal development causes transient neonatal diabetes mellitus (TNDM).

The organization of the gamma-glutamyl transferase genes and other low copy repeats in human chromosome 22q11.

J E Collins et al.

Genome research, 7(5), 522-531 (1997-05-01)

A clone map consisting of YACs, cosmids, and fosmids has been constructed covering low copy repeat regions of human chromosome 22q11. A combination of clone restriction digest analysis, single-copy landmark content analysis, HindIII-Sau3AI fingerprinting, and sequencing of PCR products derived

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